Issue 90, 2013
From the journal:

Chemical Communications

Colorimetric detection of human papilloma virus by double isothermal amplification†

Stefano Persano,a   Paola Valentini,a   Joong Hyun Kim*ab  and  Pier Paolo Pompa*a  

* Corresponding authors

a Istituto Italiano di Tecnologia (IIT), Center for Bio-Molecular Nanotechnologies@UniLe, Via Barsanti – 73010 Arnesano, Lecce, Italy
E-mail: pierpaolo.pompa@iit.it
Tel: +39-0832-1816214

b Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, South Korea
E-mail: jhkim@dgmif.re.kr

Abstract

We developed a polymerase reaction free, low-cost and sensitive assay for the colorimetric detection of Human Papilloma Virus (HPV), based on the use of a smart design exploiting magnetic microbeads, chimeric RNA/DNAzyme oligonucleotides, and double signal amplification. This method allows obtaining a fast response with a detection limit of 10 pM, avoiding the amplification of the target via traditional PCR.

Graphical abstract: Colorimetric detection of human papilloma virus by double isothermal amplification

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Article information

DOI
https://doi.org/10.1039/C3CC45459B
Article type
Communication
Submitted
18 Jul 2013
Accepted
22 Sep 2013
First published
23 Sep 2013

Chem. Commun., 2013,49, 10605-10607

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Colorimetric detection of human papilloma virus by double isothermal amplification

S. Persano, P. Valentini, J. H. Kim and P. P. Pompa, Chem. Commun., 2013, 49, 10605 DOI: 10.1039/C3CC45459B

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