Issue 96, 2013
From the journal:

Chemical Communications

Alkynyl monosaccharide analogues as a tool for evaluating Golgi glycosylation efficiency: application to Congenital Disorders of Glycosylation (CDG)†

Jorick Vanbeselaere,ab   Dorothée Vicogne,ab   Gert Matthijs,c   Christophe Biot,ab   François Foulquier‡*ab  and  Yann Guerardel‡*ab  

* Corresponding authors

a Université Lille1, UGSF, F-59650 Villeneuve d'Ascq, France
E-mail: yann.guerardel@univ-lille1.fr, francois.foulquier@univ-lille1.fr
Fax: 33 03 20 43 65 55
Tel: 33 03 20 43 48 83

b CNRS, UMR 8576, F-59650 Villeneuve d'Ascq, France

c Laboratory for Molecular Diagnostics, Center for Human Genetics, University of Leuven, B-3000 Leuven, Belgium

Abstract

The visualization of Golgi glycosylation defects in patients' cells with Congenital Disorders of Glycosylation (CDG) is challenging and necessitates the use of cumbersome glycan analysis methods that are barely adapted to clinical research. We show here that metabolic labelling of patient cells with alkyne-tagged sialic-acid (SiaNAl) enables an easy and reliable readout assay for the detection of CDG occurrence. It also provides valuable clues regarding the pathological processes by assessing the distribution of sialic acid analogues within the cells.

Graphical abstract: Alkynyl monosaccharide analogues as a tool for evaluating Golgi glycosylation efficiency: application to Congenital Disorders of Glycosylation (CDG)

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Article information

DOI
https://doi.org/10.1039/C3CC45914D
Article type
Communication
Submitted
02 Aug 2013
Accepted
05 Oct 2013
First published
09 Oct 2013

Chem. Commun., 2013,49, 11293-11295

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Alkynyl monosaccharide analogues as a tool for evaluating Golgi glycosylation efficiency: application to Congenital Disorders of Glycosylation (CDG)

J. Vanbeselaere, D. Vicogne, G. Matthijs, C. Biot, F. Foulquier and Y. Guerardel, Chem. Commun., 2013, 49, 11293 DOI: 10.1039/C3CC45914D

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