Liver, Pancreas, and Biliary TractChromosomal changes and clonality relationship between primary and recurrent hepatocellular carcinoma☆,☆☆
Section snippets
Clinical HCC samples
Thirty-one pairs of primary and recurrent HCC from the same patients were collected either from Taichung Veteran General Hospital or National Taiwan University Hospital, Taipei, between 1990 and 1997 (Table 1).Their serologic markers for HBV and HCV infections and α-fetoprotein levels were recorded. After the first curative surgery, the patients were followed up in the outpatient clinic every 1–2 months. Blood chemistry levels, liver function profiles, and α-fetoprotein levels were checked.
Major genomic changes among initial HCC tumors
Thirty-one pairs of initial and recurrent HCC collected from the same patients were analyzed by CGH. Detailed aberration results are shown in Table 1. Among all initial tumors, gain aberrations were detected frequently on chromosome arms 1q (77%), 6p (48%), 8q (45%), 17q (32%), 20q (26%), and 11q (23%). The minimal overlapping regions were at 1q21–23, 6p21–23, 8q24, 17q21, 20q13, and 11q13, respectively. Loss abnormalities were frequently found on chromosome arms 13q (64%), 4q (48%), 8p (39%),
Discussion
CGH is a powerful genetic technique for screening of chromosomal aberrations in a whole genome scale. Informative molecular and cytogenetic data have been obtained since the method was first described in 1992.21 In the present study, we assessed the use of CGH profiles as “tumor genome fingerprints” based on the reasoning that tumor tissues originated from a single clone and that most genomic defects were inherited by its entire progeny. Specifically, we used CGH data as a clonality marker to
Acknowledgements
The authors thank Dr. Kwang-Dong Wuu (National Yang-Ming University) and Prof. Ding-Shinn Chen (National Taiwan University) for their inspiration and valuable comments, and Mei-Chi Lee, Wen-Ping Tseng, Weber Chen, and Eugene Chu for their superior technical assistance and manuscript editing.
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2020, Journal of HepatologyCitation Excerpt :Another type of genomic heterogeneity observed in HCC is inter-tumour heterogeneity that can be related to de novo independent carcinogenesis on a background of cirrhosis and/or intrahepatic metastasis (Fig. 4). According to the study and the method used to assess tumour clonality, intrahepatic metastases are observed in 20 to 40% of patients, whereas the rest of the tumours are related to de novo carcinogenesis on cirrhosis.65,93–95 However, we must underline that most of these studies have been performed on surgical samples following resection or transplantation and that results may be different in more advanced stages.
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Address requests for reprints to: Chi-Hung Lin, M.D., Ph.D., Institute of Microbiology and Immunology, National Yang-Ming University, 155, Li-Non Street, Section 2, Taipei, Taiwan 112, Republic of China. e-mail: [email protected]; fax: (886) 2-28212880.
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Supported by grant NCS 89-2318-B-010-002-M51 from Frontier Medical Genomic Program, National Science Council; grant DD01-86IX-MG-607S from National Health Research Institute Contract Research; grant 89-B-FA22-2-4 from the Program for Promoting Academic Excellence of Universities (to M.-T.H.); and grants from the Yen Tjing Ling Medical Foundation and Chien-Tien Hsu Cancer Research Foundation.