Basic-alimentary tractActinin-4 increases cell motility and promotes lymph node metastasis of colorectal cancer
Section snippets
Antibodies
Anti-hemagglutinin (HA) rat monoclonal antibody (clone 3F10) was purchased from Roche Diagnostics GmbH (Mannheim, Germany). Anti-actin mouse monoclonal antibody (clone AC-15) was purchased from Abcam Inc (Cambridge, England). Anti-E-cadherin mouse monoclonal HECD1 and anti-actinin-4 rabbit polyclonal (Ab-1) antibodies were produced in our laboratory, as described previously.16, 23 Another anti-actinin-4 polyclonal (Ab-2) antibody was newly raised against the synthetic peptide NQSYQYGPSSAGNGAGC
Increased expression of actinin-4 protein in colorectal cancer
The expression of actinin-4 protein was examined in 26 cases of colorectal carcinoma using immunofluorescence histochemistry (Figure 1). The expression level of actinin-4 was increased in the cancer cells when compared with normal intestinal epithelial cells in the same specimens (Figure 1A and B). We measured the fluorescence intensity along lines of the same length placed on 3 randomly selected normal colonic crypts and 3 cancerous glands in each case and found that the expression of
Discussion
Cell movement can be viewed as a dynamic multistep cycle of (1) extension of podia, (2) attachment of the leading edge of podia to the substrate, (3) forward retraction of the cell body, and (4) detachment of rear adhesions.30 Although the molecular mechanisms of podia formation are not perfectly understood, a certain set of actin-binding proteins seems to be involved, because filamentous actin is highly concentrated in the leading edge of podia.10, 11, 12, 13 In this study, we showed that DLD1
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Supported by grants from the Ministry of Health, Labor and Welfare and by the Program for the Promotion of Fundamental Studies in Health Sciences of the Organization for Pharmaceutical Safety and Research of Japan. Y. H. is a recipient of the Research Resident Fellowship from the Foundation for the Promotion of Cancer Research.