Clinical–alimentary tractRare CpG Island Methylator Phenotype in Ulcerative Colitis–Associated Neoplasias
Section snippets
Patient Samples
We examined a total of 69 primary neoplasias (48 cancers and 21 dysplasias) from 65 patients with UC. All tissue specimens were obtained from patients with UC undergoing surgery at the Mount Sinai Medical Center. The dates of tissue sample acquisition for cancers were similar to those for dysplasias. A series of 69 S-CRCs from 69 patients undergoing surgery at the Johns Hopkins Hospital (n = 31) and the MD Anderson Cancer Center (n = 38) were used as controls. Forty-six samples of colonic
Clinicopathologic Characteristics of Patients
Table 1 shows the clinicopathologic characteristics of studied patients with UC-associated dysplasias (UC-Ds), UC-associated cancers (UC-Cs), and S-CRCs. There was no significant difference in any clinicopathologic characteristics except patient age between UC-Ds or S-CRCs and UC-Cs. Patients with UC-Cs and UC-Ds were significantly younger than those with S-CRCs (age: 50.6 y in UC-Ds and 53.3 y in UC-Cs vs 68.1 y in S-CRC, P = .001 and P < .001, respectively).
Methylation Status in UC-C and S-CRC
The methylation status of 10 genes
Discussion
Previous reports have suggested that epigenetic alterations in UC-associated neoplasias are different from those in sporadic colorectal neoplasias.37, 38, 39, 40 However, this issue remains controversial and different methodology may have contributed to the discrepant results obtained. Here, using a quantitative methylation analysis method, we clearly show that UC-Ds have a high degree of age-related methylation abnormalities as previously reported, but unexpectedly also show that UC-Cs have
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Molecular basis of diseases of the gastrointestinal tract
2018, Molecular Pathology: The Molecular Basis of Human DiseaseThe molecular landscape of colitis-associated carcinogenesis
2017, Digestive and Liver DiseaseCitation Excerpt :MLH1 promoter hypermethylation is less frequent than in sporadic MSI cancers, however, because oxidative stress and ROS might cause MSI even in the absence of defects in the DNA mismatch repair pathway [29]. The CIMP pathway is also seen less often in CAC [80,81]. For the last three decades, microRNAs (miRNAs) have represented the most promising class of diagnostic and prognostic biomarkers.
Supported in part by National Institutes of Health grants CA098006 and CA105346 (to J.P.J. Issa) and CA95323 and CA77057 (to S.J. Meltzer).
- 1
Dr Meltzer’s current affiliation is Gastroenterology Division, Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD
- 2
Dr Konishi’s current affiliation is Second Department of Internal Medicine, Showa University School of Medicine, Tokyo, Japan.