Clinical–Alimentary TractMultigene Analysis Can Discriminate Between Ulcerative Colitis, Crohn's Disease, and Irritable Bowel Syndrome
Section snippets
Pilot study
Biopsy samples were collected in a prospective manner at the IBD-unit at Sophiahemmet (Stockholm, Sweden). Fifty-five IBD patients (23 women and 32 men) with a mean age of 44.6 years (range, 19–76 y), and a mean disease duration of 4.5 years (range, 1 mo to 18 y) and 8 IBS patients were included.
Retrospective study
Complementary DNA (cDNA) samples derived from mucosal biopsy samples of 55 IBD and 88 non-IBD patients were used (65 women and 78 men), collected at the Sahlgrenska University Hospital (Gothenburg,
Screening for IBD-Specific Genes
To identify IBD-specific genes we used a screening procedure with the subtractive suppression hybridization method, which enables the enrichment and isolation of dysregulated genes by comparing 2 cDNA populations,10, 12 in this case inflamed and noninflamed biopsy samples of 8 UC patients. For each patient, 2 subtractions were performed, enabling the isolation of both up-regulated and down-regulated genes specific to the inflamed biopsy specimens (Figure 1A). This resulted in the identification
Discussion
Gastrointestinal disorders such as IBD can dramatically affect quality of life,17 and the diagnosis can be a challenging process. In up to 30% of patients, only a temporary diagnosis of IBDU can be made. An early and accurate assessment of the diagnosis could have important clinical implications in the choice of medical treatment and for surgery considerations (eg, when colectomy, and later construction of a pelvic pouch, will be contemplated). Genetic and serologic studies have shown
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Supported by a grant from the foundation for clinical research at Sophiahemmet, Stockholm, Sweden.