Original ResearchBasic and Translational—Alimentary TractGlobal and Deep Molecular Analysis of Microbiota Signatures in Fecal Samples From Patients With Irritable Bowel Syndrome
Section snippets
Participants
The study group consisted of 62 primary care IBS patients and 46 healthy subjects, all recruited from the Helsinki area in Finland for 2 follow-up clinical trials that recruited IBS patients17 and healthy subjects.18 IBS patients fulfilled the Rome II criteria,19 were recruited in primary health care centers, and had a clinical investigation with colonoscopy or barium enema of the colon performed during the preceding 5 years. The IBS patients were divided across 3 subgroups:
Phylogenetic Profiling and Multivariate Analysis of the Total Fecal Microbiota
The microarray datasets of the fecal microbiota derived from 62 IBS patients and 46 healthy subjects were acquired and hierarchically clustered based on the signal intensity of the 3699 distinct HITChip oligonucleotide probes (Figure 1). The IBS and healthy subjects did not cluster significantly according to their health status, testifying for the individuality of the microbiota when analyzed at the phylotype level. The microbiota profiles were compared by using the average hybridization signal
Discussion
The composition of the total fecal microbiota of the 62 IBS patients and 46 healthy controls was found to be strongly and significantly influenced by the health status of the subjects. A clear and consistent multivariate separation was obtained with all of the more than 100 analyzed fecal samples into an IBS or healthy group (Figure 2). This extends similar observations made with pooled fecal DNA samples of about 50 subjects11 because the present study shows the global microbiota analysis of
Conclusions
We have presented the results of a comprehensive analysis using high throughput DNA technologies that showed specific and robust signatures in the fecal microbiota of IBS patients, some of which were characteristic for the studied IBS subtypes. These signatures provide a molecular basis for designing specific detection approaches. Despite the fact that the fecal microbiota is an extremely complex ecosystem varying significantly among individuals, most of the deviations reported here accommodate
References (46)
- et al.
Functional bowel disorders
Gastroenterology
(2006) Inflammation as a basis for functional GI disorders
Best Pract Res Clin Gastroenterol
(2004)- et al.
Post-infectious irritable bowel syndrome
Gastroenterology
(2009) - et al.
The relationship between intestinal microbiota and the central nervous system in normal gastrointestinal function and disease
Gastroenterology
(2009) - et al.
The fecal microbiota of irritable bowel syndrome patients differs significantly from that of healthy subjects
Gastroenterology
(2007) - et al.
Activation of human enteric neurons by supernatants of colonic biopsy specimens from patients with irritable bowel syndrome
Gastroenterology
(2009) - et al.
Studies on amine production in the human colon: enumeration of amine forming bacteria and physiological effects of carbohydrate and pH
Anaerobe
(1996) - et al.
A serpin from the gut bacterium Bifidobacterium longum inhibits eukaryotic elastase-like serine proteases
J Biol Chem
(2006) - et al.
Abnormal colonic fermentation in irritable bowel syndrome
Lancet
(1998) - et al.
Irritable bowel syndrome in twins: genes and environment
Gut
(2006)
Abnormal intestinal permeability in subgroups of diarrhea-predominant irritable bowel syndromes
Am J Gastroenterol
High-throughput diversity and functionality analysis of the gastrointestinal tract microbiota
Gut
Review article: Probiotics and prebiotics in irritable bowel syndrome
Aliment Pharmacol Ther
Rifaximin therapy for patients with irritable bowel syndrome without constipation
N Engl J Med
The host genotype affects the bacterial community in the human gastrointestinal tract
Microbial Ecol Health Dis
Gastrointestinal microbiota in irritable bowel syndrome: present state and perspectives
Microbiology
Diversity of the human gastrointestinal tract microbiota revisited
Environ Microbiol
Development and application of the human intestinal tract chip, a phylogenetic microarray: analysis of universally conserved phylotypes in the abundant microbiota of young and elderly adults
Environ Microbiol
Comparative analysis of pyrosequencing and a phylogenetic microarray for exploring microbial community structures in the human distal intestine
PLoS One
Enterotypes of the human gut microbiome
Nature
Multispecies probiotic supplementation alleviates the symptoms of irritable bowel syndrome and stabilises intestinal microbiota
Aliment Pharmacol Ther
Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults
World J Gastroenterol
Functional bowel disorders and functional abdominal pain
Gut
Cited by (812)
Studies of peach gum polysaccharide on gut microbiota in vitro fermentation by human feces
2025, Journal of Future FoodsPolystyrene microplastics disrupted physical barriers, microbiota composition and immune responses in the cecum of developmental Japanese quails
2024, Journal of Environmental Sciences (China)Potential effects of sodium hyaluronate on constipation-predominant irritable bowel syndrome
2024, International ImmunopharmacologyGender-specific insights into the irritable bowel syndrome pathophysiology. Focus on gut dysbiosis and permeability
2024, European Journal of Internal Medicine
Conflicts of interest The authors disclose no conflicts.
Funding This work was partially funded by the unrestricted Spinoza Award of the Netherlands Organization of Scientific Research awarded to WMdV.