Planta Med 2014; 80 - PD15
DOI: 10.1055/s-0034-1382436

Isolation of new polyacetylene from atractylodes lancea rhizome and evaluation of lipase inhibition and anti-obesity effect

P Jiao 1, J Tseng-Crank 1, B Corneliusen 1, M Yimam 1, M Hodges 1, L Brownell 1, M Hong 1, C Maurseth 1, M Chu 1, 2, M Oh 2, H Kim 2, E Hyun 1, 2, Q Jia 1, 2
  • 1Unigen USA, 3005 1st Avenue, Seattle, WA 98121, USA
  • 2Unigen Korea, 200 – 1 Songjung-Ri, Byeongcheon-Myeon, Cheonam-Si, Chungnam 330 – 863, South Korea

The ethanol extract of Atractylodes lancea rhizome displayed significant lipase inhibition with an IC50 value of 9.06 µg/mL in human pancreatic lipase assay from high-throughput screening. Bioassay-guided isolation led to the identification of one new polyacetylene syn-(5E,11E)-3-acetoxy-4-O-(3-methylbutanoyl)-1,5,11-tridecatriene-7,9-diyne-3,4-diol (7) along with six known compounds (1 – 6). The structure of compound 7 was determined based on analysis of NMR and MS data. Among these seven lipase inhibitors, the major compound atractylodin (1) showed the highest lipase inhibitory activity (IC50= 39.12µM). The anti-obesity effect of the ethanol extract of Atractylodes lancea rhizome (AL-EE) in a high-fat diet induced obesity (DIO) mice model at daily dosages of 250 mg/kg and 500 mg/kg body weight will also be presented.