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DOI: 10.1055/s-0034-1382436
Isolation of new polyacetylene from atractylodes lancea rhizome and evaluation of lipase inhibition and anti-obesity effect
The ethanol extract of Atractylodes lancea rhizome displayed significant lipase inhibition with an IC50 value of 9.06 µg/mL in human pancreatic lipase assay from high-throughput screening. Bioassay-guided isolation led to the identification of one new polyacetylene syn-(5E,11E)-3-acetoxy-4-O-(3-methylbutanoyl)-1,5,11-tridecatriene-7,9-diyne-3,4-diol (7) along with six known compounds (1 – 6). The structure of compound 7 was determined based on analysis of NMR and MS data. Among these seven lipase inhibitors, the major compound atractylodin (1) showed the highest lipase inhibitory activity (IC50= 39.12µM). The anti-obesity effect of the ethanol extract of Atractylodes lancea rhizome (AL-EE) in a high-fat diet induced obesity (DIO) mice model at daily dosages of 250 mg/kg and 500 mg/kg body weight will also be presented.