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Int J Sports Med 2000; 21(4): 289-293
DOI: 10.1055/s-2000-13308
Immunology
Georg Thieme Verlag Stuttgart ·New York

Influence of an Acute Exercise on Neutrophil and Platelet Adhesion, Nitric Oxide Plasma Metabolites in Inactive and Active Subjects

L. Cuzzolin1 , S. Lussignoli3 , F. Crivellente1 , A. Adami1 , F. Schena2 , P. Bellavite3 , G. Brocco3 , G. Benoni1
  • 1 Dept. of Medicine & Public Health - Pharmacology, University of Verona, Verona, Italy
  • 2 E.C.U.S. Interuniversitary Center for Sport Sciences, Rovereto, Italy
  • 3 Dept. of Morphological & Biomedical Science - Chemical and Clinical Microscopy, University of Verona, Verona, Italy
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Publication History

Publication Date:
31 December 2000 (online)

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In this work we studied the influence of an acute exercise either on nitrite/nitrate plasma levels or on neutrophil and platelet adhesion in inactive and active subjects. Twelve healthy subjects (6 inactives and 6 actives) exercised on a racing cycle ergometer performing stepwise increases in intensity until reaching, within 5 min, a heart rate of 150 beats × min−1 which represents an oxygen consumption of about 75 % of the individual maximum rate of oxygen uptake. From peripheral venous blood samples (drawn from all subjects before, immediately after the end of exercise, and 1 hour later) neutrophils and platelets were isolated to test plate adhesion, and nitrite/nitrate concentrations were measured in the plasma. Immediately after the acute exercise, in active subjects we observed a significant decrease in the percentage of neutrophil adhesion (7.96 ± 2.38 vs. 14.10 ± 3.14), associated with an increase in nitrite/nitrate plasma levels (81.38 ± 10.76 vs. 41.08 ± 8.13 μmol × l−1), restored by a 40 min pre-incubation with NG-nitro-L-arginine methyl ester (L-NAME). In unstimulated platelets we observed a significant lower percentage of platelet adhesion in active subjects compared to inactives after exercise. With thrombin or adenosine 5′-diphosphate as agonists platelet adhesion did not result significantly different in active subjects compared to inactives. In conclusion, our data show that physical exercise can induce changes in some cell activities, even if transient, and favour the generation of nitric oxide. The lower adhesion of neutrophils and platelets induced by regular exercise could be an important goal in the prevention of vascular and inflammatory diseases.

Key words:

Physical exercise - nitric oxide - cell functions

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Dr. Giuseppina Benoni

Institute of Pharmacology

University of Verona

Policlinico Borgo Roma

37134 Verona

Italy

Phone: + 39 (45) 8098609

Fax: + 39 (45) 581111

Email: Laura@Farma.univr.it

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