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Horm Metab Res 2001; 33(9): 520-524
DOI: 10.1055/s-2001-17209
Original Basic

© Georg Thieme Verlag Stuttgart · New York

Increased Hypothalamic Expression of the p75 Tumor Necrosis Factor Receptor in New Zealand Obese Mice

N. M. Rizk 1 , H.-G. Joost 2 , J. Eckel 3
  • 1 Physiology Department, Mansoura Faculty of Medicine, Mansoura University, Egypt
  • 2 Institute of Pharmacology and Toxicology, RWTH Aachen, Germany
  • 3 Molecular Cardiology, German Diabetes Research Institute, Düsseldorf, Germany
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Publication History

Publication Date:
17 September 2001 (online)

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Previous studies have demonstrated that tumor necrosis factor-α (TNF-α) production from adipose tissue is elevated in obese animal models and in obese humans. It plays an important role in the induction of insulin resistance in experimental animals. In this study, we examined hypothalamic tissue expression of TNF-α and its receptors and TNF-α expression of adipose tissue in lean C57BLKSJ+/+ and obese polygenic New Zealand obese (NZO) mice. Obese animals exhibited hyperglycemia, hyperinsulinemia, hypertriglyceridemia, and hypercholesterinemia. Using RT-PCR, we observed increased expression (2.4-fold) of TNF receptor 2 (p75) in the hypothalamus of obese mice. TNF-α expression in adipose tissue of obese mice was eight times higher than in controls. TNF-α and TNF receptor 1 (p55) expression in hypothalamic tissue was similar in obese and lean animals. These results suggest that the hypothalamic TNF receptor 2 (p75) might play a role in obesity by modulating the actions of TNF-α in conditions of leptin resistance.

Key words:

TNF-alpha - TNF Receptors - New Zealand Obese Mouse - Obesity - Hypothalamus

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Prof. Dr. J. Eckel

German Diabetes Research Institute

Auf’m Hennekamp 65
40225 Düsseldorf
Germany


Phone: + 49 (211) 3382 561

Fax: + 49 (211) 3382 561

Email: eckel@uni-duesseldorf.de

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