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DOI: 10.1055/s-2004-818939
© Georg Thieme Verlag Stuttgart · New York
Isolation of a New 15-Membered Macrocyclic Glycolipid Lactone, Cuscutic Resinoside A from the Seeds of Cuscuta chinensis: A Stimulator of Breast Cancer Cell Proliferation
This work was supported by Health Sciences Research Grants for Research on Environmental Health from the Ministry of Health and Welfare, JapanPublication History
Received: September 10, 2003
Accepted: December 12, 2003
Publication Date:
19 April 2004 (online)
Abstract
While searching for new estrogenic compounds from the plant kingdom, we investigated an extract of the seeds of Cuscuta chinensis (Convolvulaceae) which showed potency for stimulating MCF-7 cell proliferation. A novel resin glycoside, cuscutic resinoside A (6) was isolated along with five known compounds from the extract. The structure was deduced from its spectral data as (11S)-hydroxyhexadecanoic acid 11-O-α-L-(4-O-2R,3R-nilylrhamnopyranosyl)-(1→2)-O-α-L-rhamnopyranosyl-(1,2-lactone) forming a unique 15-membered macrocyclic lactone. The compound significantly stimulated not only MCF-7 cell proliferation but also T47D human breast cancer cells at a concentration of 10 μM. Along with cuscutamine (1) and kaempferol (4), 6 was tested in the transient luciferase reporter assay and was found to have different luciferase inducing activity characteristics from the other compounds. These results suggest that 6 stimulated cancer cell proliferation by a different mechanism from 1 and 4.
Key words
MCF-7 cells - T47D cells - phytoestrogen - luciferase - Cuscuta chinensis - Convolvulaceae - seeds - resin glycoside
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Dr. Kaoru Umehara
School of Pharmaceutical Sciences
University of Shizuoka
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Japan
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Email: umehara@ys7.u-shizuoka-ken.ac.jp