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Horm Metab Res 2004; 36(8): 564-570
DOI: 10.1055/s-2004-825762
Original Clinical
© Georg Thieme Verlag Stuttgart · New York

Novel Resistin Promoter Polymorphisms: Association with Serum Resistin Level in Japanese Obese Individuals

K.  Azuma1 , S.  Oguchi2 , Y.  Matsubara1 , T.  Mamizuka2 , M.  Murata1, 2 , H.  Kikuchi2 , K.  Watanabe2 , F.  Katsukawa3 , H.  Yamazaki3 , A.  Shimada1 , T.  Saruta1
  • 1Department of Internal Mecicine, School of Medicine
  • 2Department of Laboratory Medicine, School of Medicine
  • 3Sports Medicine Research Center, Keio University, Tokyo, Japan
Further Information

Publication History

Received 24 November 2003

Accepted after Revision 1 March 2004

Publication Date:
24 August 2004 (online)

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Abstract

Objectives: Resistin, an adipocyte-secreted cytokine recently discovered in mice, has been proposed as a link between obesity and diabetes. We analyzed resistin gene polymorphisms and examined their association with serum resistin level and obesity phenotypes in humans. Subjects and Methods: Sixty young, obese, non-diabetic subjects taking no medication were studied. DNA sequencing and genotyping of identified single nucleotide polymorphisms were performed. Associations between polymorphisms and serum resistin level, BMI, body composition, fat distribution, and several indices of insulin sensitivity were examined. Moreover, single nucleotide polymorphisms in the promoter region were examined for their influence on resistin gene transcriptional activity using luciferase reporter vectors. Results: Ten non-coding single nucleotide polymorphisms were found. The -638G>A, -420C>G, and -358G>A polymorphisms in the promoter region showed marked linkage disequilibrium with each other, and were associated with serum resistin level; however, there was no association between these polymorphisms and parameters related to adiposity or insulin resistance. The results of luciferase assay revealed that -638G>A together with the -420C>G polymorphism influenced resistin gene transcriptional activity. Conclusion: We found that variability in the serum resistin level might be related to polymorphic variants of the promoter region of the gene.

Kew words

Resistin - Single nucleotide polymorphism - Serum level - Luciferese assay - Adiposity - Insulin resistance

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K. Azuma

Division of Endocrinology and Metabolism, Department of Internal Medicine, Keio University School of Medicine

35 Shinanomachi · Shinjuku-ku · Tokyo 160-8582 · Japan

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