Plasma Protein Carbonyl Response to Increasing Exercise Duration in Aerobically Trained Men and Women

 

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Abstract

The purpose of this study was to determine the effect of aerobic exercise duration on plasma protein carbonyl concentrations, a marker of protein oxidation, in aerobically trained men and women. Eight men (age: 27 ± 4 years, V·O_2peak: 4.09 ± 0.26 L · min^-1; mean ± SD) and 7 women (age: 27 ± 6 years, V·O_2peak: 2.33 ± 0.24 L · min^-1) exercised on an electrically-braked cycle ergometer at 70 % V·O_2peak for 30, 60 or 120 minutes on three separate days. Plasma samples collected before and immediately, 30- and 60-minutes post-exercise were analyzed for protein carbonyls. Mean oxygen uptake was greater for men in all conditions (2.75 ± 0.03 L · min^-1; 38 ± 0.43 ml · kg^-1 · min^-1) compared to women (1.57 ± 0.03 L · min^-1; 24.1 ± 0.47 ml · kg^-1 · min^-1). Total work performed during the exercise sessions was also greater for men than for women during the 30 (368 ± 11 versus 223 ± 7 kJ), 60 (697 ± 17 versus 423 ± 18 kJ), and 120-minute conditions (1173 ± 44 versus 726 ± 28 kJ) (Mean ± SEM). Although these comparisons were significant (p < 0.0001), sex differences in total work performed and mean V·O₂ did not result in sex differences in protein carbonyls. However, a condition by time interaction was observed with greater post-exercise values following the 120-minute condition compared to both the 30- and 60-minute conditions. Protein carbonyl concentration was greatest immediately post-exercise for both men and women and generally declined in a linear trend through one hour of recovery. These data suggest that protein carbonyl concentration is elevated by cycling exercise performed at 70 % V·O_2peak, is greater following longer duration rides, begins to recover within one hour following exercise, and is not different between men and women.

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Laurie Wideman

Department of Exercise and Sport Science
237E HHP Building
University of North Carolina at Greensboro

Greensboro, NC 27402 - 6169

USA

Phone: + 33 63 34 32 34

Fax: + 33 63 34 32 38

Email: l_widema@uncg.edu