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Horm Metab Res 1994; 26(10): 465-469
DOI: 10.1055/s-2007-1001734
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© Georg Thieme Verlag, Stuttgart · New York

Identification of δ-Iodolactone in Iodide Treated Human Goiter and its Inhibitory Effect on Proliferation of Human Thyroid Follicles

A. Dugrillon, W. M. Uedelhoven, M. A. Pisarev, G. Bechtner, R. Gärtner
  • Medizinische Klinik and Institut für Prophylaxe der Kreislauferkrankungen, Klinikum Innenstadt, Universität München, München, Germany
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Publication History

1993

1994

Publication Date:
14 March 2008 (online)

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Summary

There is evidence that iodoarachidonates are mediators of iodide in thyroid autoregulation, however, their occurrence in vivo has not yet been demonstrated. We therefore tried to identify δ-iodolactone (5-Hydroxy-6-iodo-8,11,14-eicosatrienoic δ-lactone, IL-δ) in thyroid tissue from a patient with Graves' disease treated with high doses of iodide. Lipids were extracted from thyroid tissue, purified by reversed phase chromatography and analyzed by gas chromatography - tandem mass spectrometry (GC-MSMS). The retention time in gas chromatography and fragmentation pattern in tandem mass spectrometry were determined with biochemically synthesized non-deuterated and deuterated IL-δ. According to retention time (13.44 min) and specific fragments (m/z 303, m/z 259) the occurrence of IL-δ could be demonstrated in the extract of iodide treated goiter. In vitro, potassium iodide (40 μM) as well as IL-δ (1.0 μM) significantly inhibited the proliferation of human thyroid follicular cells induced by phorbol ester TPA (12-O-tetradecanoylphorbol 13-acetate). These results demonstrate for the first time that Il-δ is present in iodide treated human thyroid. As cell proliferation is under negative control of IL-δ, a crucial role in thyroid involution following iodide treatment may be possible.

Key words

Iodide - Iodolactone - GC-MS - Thyroid Growth - Autoregulation

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