Original ArticlesHuman skin expresses growth hormone but not the prolactin gene☆,☆☆
Section snippets
Human tissue
Fresh human skin used for RNA isolation was obtained from tissue discarded after surgical procedures at Albany Medical College or affiliated offices.25 Preparations of RNA were stored at –80°C until the time of analysis. Samples from previously diagnosed skin biopsy samples, including lesional and non-lesional specimens, were cut from paraffin-embedded blocks and used for immunocytochemical analyses. Control experiments were performed in PBMCs obtained from 2 normal female subjects, ages 30 and
Results and discussion
The expression of GH and PRL has been reported previously in dermal fibroblasts.23, 29 We investigated the extent of that expression by testing for the corresponding genes in whole human skin and in cultured epidermal cells. Fig 1 shows the amplification of cDNAs obtained from human melanoma cells (Fig 1, lane 1 ), hamster melanoma cells (Fig 1, lane 2) , HaCaT keratinocytes (Fig 1, lane 3 ), and human PBMCs (Fig 1, lane 4 ).
Acknowledgements
We thank Ms Carolyn Cheney for technical help.
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Supported by National Institutes of Health Grant M001RR00034 from the General Clinical Research Center (W.B.M), by Grant IBN-9896030 from the National Science Foundation (A.S.), and by Grant 99-51 from the American Cancer Society, Illinois Division (A.S.).
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Reprint requests: Andrzej Slominski, MD, PhD, Department of Pathology, Room 576 BMH Main, University of Tennessee, 899 Madison Ave, Memphis, TN 38163.