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New Zealand grasslands revisited: identification of Cocksfoot mild mosaic virus

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Abstract

In the springs of 2003 and 2004, Bromus diandrus plants showing conspicuous mosaic symptoms were observed at Waitati, East Otago, New Zealand. Electron microscopic examination of sap samples in 2% phosphotungstate (pH 6.5) revealed large numbers of isometric particles 25 nm in diameter. The virus was mechanically transmitted to Setaria italica and Secale cereale cvv. Amilo and Rahu but not to Avena sativa, Chenopodium spp., Hordeum vulgare, Nicotiana benthamiana, Triticum aestivum or Zea spp. In Ouchterlony double diffusion tests, sap from infected plants and purified particles reacted strongly with antisera to Cocksfoot mild mosaic virus (CMMV and CMMV-Phleum mottle).Weaker reactions were observed with an antiserum to Panicum mosaic virus and there were no reactions with antisera to eight other sobemoviruses and ten other viruses known to infect the Poaceae. ELISA was used to test archived and fresh samples for CMMV and other viruses known to infect the Poaceae. CMMV was detected in B. diandrus and Ryegrass mosaic virus was detected in Holcus lanatus and Lolium perenne. However, Brome mosaic virus, Brome streak virus, Soilborne wheat mosaic virus, Wheat dwarf virus and Wheat streak mosaic virus were not detected in samples collected between 1995–2005 from Auckland and the South Island of New Zealand. This is the first report of CMMV in the Southern Hemisphere.

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References

  • Brunt A, Crabtree K, Dallwitz M, Gibbs A, Watson L (1996) ‘Viruses of Plants. Descriptions and Lists from the VIDE Database.’ (CAB International: Wallingford)

    Google Scholar 

  • Campbell AW, Guy PL (2001) Cocksfoot mottle virus spreads to the South Island of New Zealand. Australasian Plant Pathology 30, 217–220. doi: 10.1071/AP01027

    Article  Google Scholar 

  • Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34, 475–483.

    Article  CAS  PubMed  Google Scholar 

  • D’Arcy CJ (1995) Symptomology and host range of barley yellow dwarf. In ‘Barley yellow dwarf: 40 years of progress’. (Eds CJ D’Arcy, PA Burnett) pp. 9–28. (APS Press: St Paul)

    Google Scholar 

  • Davis LT, Guy PL (2001) Introduced plant viruses and the invasion of a native grass flora. Biological Invasions 3, 89–95. doi: 10.1023/A:1011464804245

    Article  Google Scholar 

  • Ellis MH, Rebetzke GJ, Mago R, Chu P (2003) First report of Wheat streak mosaic virus in Australia. Australasian Plant Pathology 32, 551–553. doi: 10.1071/AP03054

    Article  Google Scholar 

  • Malmstrom CM, Hughes CC, Newton LA, Stoner CJ (2005a) Virus infection in remnant native bunchgrasses from invaded California grasslands. New Phytologist 168, 217–230. doi: 10.1111/j.1469-8137.2005.01479.x

    Article  CAS  PubMed  Google Scholar 

  • Malmstrom CM, McCullough AJ, Johnson HA, Newton LA, Borer ET (2005b) Invasive annual grasses indirectly increase virus incidence in California native perennial bunchgrass. Oecologia 145, 153–164. doi: 10.1007/s00442-005-0099-z

    Article  PubMed  Google Scholar 

  • Paul HL, Huth W, Querfurth G (1973) Cocksfoot mild mosaic virus — Phleum mottle virus: a Comparison. Intervirology 2, 253–260.

    Article  Google Scholar 

  • Pearson MN, Clover GRG, Guy PL, Fletcher JD, Beever RE (2006) A review of the plant virus, viroid and mollicute records for New Zealand. Australasian Plant Pathology 35, 217–252. doi: 10.1071/AP06016

    Article  Google Scholar 

  • Smales TS (1997) Invertebrate Pests of Pasture as Potential Plant Virus Vectors. MSc thesis, University of Otago, Dunedin, New Zealand.

    Google Scholar 

  • Smales T, Ferguson CM, Guy PL, Shand JA (1996) A survey of ryegrass mosaic virus and endophyte in Otago and Southland. In ‘Proceedings of the 49th New Zealand Plant Protection Conference’. pp. 220–224.

  • Torrance L, Harrison BD (1981) Properties of Scottish Isolates of Cocksfoot mild mosaic virus and their comparison with others. Annals of Applied Biology 97, 285–295.

    Article  CAS  Google Scholar 

  • Webster DE, Beck DL, Rabenstein F, Forster RLS, Guy PL (2005) An improved polyclonal antiserum for detecting Ryegrass mosaic rymovirus. Archives of Virology 150, 1921–1926. doi: 10.1007/s00705-005-0531-z

    Article  CAS  PubMed  Google Scholar 

Download references

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  1. Botany Department, University of Otago, Box 56, 9054, Dunedin, New Zealand

    P. L. Guy

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  1. P. L. Guy
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Correspondence to P. L. Guy.

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Guy, P.L. New Zealand grasslands revisited: identification of Cocksfoot mild mosaic virus . Australasian Plant Pathology 35, 461–464 (2006). https://doi.org/10.1071/AP06048

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  • DOI: https://doi.org/10.1071/AP06048

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