Journal of Biological Chemistry
Protein Synthesis, Post-Translational Modification, and DegradationSecreted Cyclophilin A, a Peptidylprolyl cis-trans Isomerase, Mediates Matrix Assembly of Hensin, a Protein Implicated in Epithelial Differentiation*
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The abbreviations used are: ECM, extracellular matrix; SRCR, scavenger receptor cysteine-rich; SID, SRCR-interspersed domain; ZP, zona pellucida; PPIase, peptidylprolyl cis-trans isomerase; CsA, cyclosporin A; Cyp, cyclophilin; LiE, 6-(3,4-dichlorophenyl)-4-(N,N-dimethylaminoethylthio)-2-phenylpyriidine; Fmoc, N-(9-fluorenyl)methoxycarbonyl; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; CHAPS, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid; RT, reverse transcription; Ni-NTA, nickel-nitrilotriacetic acid; ANOVA, analysis of variance; HD, high density; FKBP, FK506 (tacrolimus)-binding protein.
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G. Fischer and J. Liebscher, unpublished results.
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This work was supported, in whole or in part, by National Institutes of Health Grants DK-20999 (to Q. Al-A.) and DK-50603 (to G. J. S.). This work was also supported by Grant-in-aid 0150138N from the American Heart Association (to G. J. S.) and by Deutsche Forschungsgemeinschaft Grant GK1026 (to C. S.-F.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The on-line version of this article (available at http://www.jbc.org) contains supplemental Figs. 1 and 2.
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Both authors contributed equally to this manuscript.