Paper

Pro-apoptotic NOXA is implicated in atmospheric-pressure plasma-induced melanoma cell death

, and

Published 15 October 2015 © 2015 IOP Publishing Ltd
, , Citation M Ishaq et al 2015 J. Phys. D: Appl. Phys. 48 464002 DOI 10.1088/0022-3727/48/46/464002

0022-3727/48/46/464002

Abstract

Atmospheric-pressure plasma (APP) has been successfully used to treat several types of cancers in vivo and in vitro, with the effect being primarily attributed to the generation of reactive oxygen species (ROS). However, the mechanisms by which APP induces apoptosis in cancer cells require further elucidation. In this study, the effects of APP on the expression of 500 genes in melanoma Mel007 cancer cells were examined. Pro-apoptotic phorbol-12-myristate-13-acetate-induced protein (PMAIP1), also known as NOXA, was highly expressed as a result of APP treatment in a dose-dependent manner. Blocking of ROS using scavenger NAC or silencing of NOXA gene by RNA interference inhibited the APP-induced NOXA genes upregulation and impaired caspases 3/7 mediated apoptosis, confirming the important role plasma-generated ROS species and pro-apoptotic NOXA play in APP-induced cancer cell death.

Export citation and abstract BibTeX RIS

Access this article

The computer you are using is not registered by an institution with a subscription to this article. Please choose one of the options below.

Login

IOPscience login

Find out more about journal subscriptions at your site.

Purchase from

Article Galaxy
CCC RightFind

Purchase this article from our trusted document delivery partners.

Make a recommendation

To gain access to this content, please complete the Recommendation Form and we will follow up with your librarian or Institution on your behalf.

For corporate researchers we can also follow up directly with your R&D manager, or the information management contact at your company. Institutional subscribers have access to the current volume, plus a 10-year back file (where available).

Please wait… references are loading.