Basic Research-TechnologyThe Susceptibility of Starved, Stationary Phase, and Growing Cells of Enterococcus faecalis to Endodontic Medicaments
Section snippets
Bacteria and Culture Conditions
The strains of E. faecalis used in this study were strain VP3-80 isolated from root filled tooth with chronic apical periodontitis in Lithuania (4) and strain A197A isolated from a case of persistent apical periodontitis in Finland (3). Cultures of the E. faecalis strains were grown overnight at 37°C on tryptic-soy-agar plates (TSA; Difco Laboratories, Detroit, MI). Identification of the strains has been described earlier by Sirén et al. (3) and Peciuliene et al. (8).
The growth curve was
Growth Curve
The amount of living cells increased exponentially during the first 6 h of incubation (exponential growth phase, Fig. 2). Between 6 and 24 h the amount of living cells remained stable (stationary phase); it decreased between 24 and 36 h and remained stable thereafter (starvation phase). The optical density measured at the respective time intervals increased during the first 12 h and remained unchanged thereafter.
Electrophoresis of E. faecalis Proteins
SDS-page patterns of proteins extracted from E. faecalis cells grown for 3, 12, and
Discussion
The main goal of the study was to test the susceptibility of bacterial cells in different physiological stages to three different disinfectants commonly used in endodontics. It was shown that the resistance of E. faecalis cells to the tested medicaments increased dramatically in aging cultures. Recent studies with E. faecalis have shown that cells growing in rich media are more susceptible to different types of stress than glucose-starved cells (24., 26.). There seems to be no corresponding
Acknowledgments
The authors thank Britt-Mari Kvam for technical assistance. The authors also thank Steinar Stølen and Renate Hars for the help with scanning and transmission electron microscopy.
References (33)
- et al.
Kinetics of immune cell and bone resorptive responses to endodontic infections
J Endod
(1992) - et al.
Polymerase chain reaction-based analysis of microorganisms associated with failed endodontic treatment
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
(2004) - et al.
Association of Enterococcus faecalis with different forms of periradicular diseases
J Endod
(2004) - et al.
Isolation of Enterococcus faecalis in previously root-filled canals in a Lithuanian population
J Endod
(2000) - et al.
Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative re-treatment
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
(1998) - et al.
Antimicrobial evaluation of calcium hydroxide in infected dentinal tubules
J Endod
(1999) - et al.
pH required to kill Enterococcus faecalis in vitro
J Endod
(2004) - et al.
Additive antimicrobial activity of calcium hydroxide and chlorhexidine on common endodontic bacterial pathogens
J Endod
(2003) - et al.
Antibacterial efficacy of a new chlorhexidine slow release device to disinfect dentinal tubules
J Endod
(2003) - et al.
In vitro antimicrobial activity of various medication preparations on E. faecalis in root canal dentin
J Endod
(2003)
Inactivation of the antibacterial activity of iodine potassium iodide and chlorhexidine digluconate against Enterococcus faecalis by dentin, dentin matrix, type-I collagen, and heat-killed microbial whole cells
J Endod
An effective method of inactivating chlorhexidine
Oral Surg Oral Med Oral Pathol Oral Radiol Endod
Microbiological status of root-filled teeth with apical periodontitis
Int Endod J
Microbiological findings and clinical treatment procedures in endodontic cases selected for microbiological investigation
Int Endod J
Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis
Int Endod J
Microorganisms from canals of root-filled teeth with periapical lesions
Int Endod J
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