1887

Abstract

We formally propose the name sp. nov. for a new spotted fever group (SFG) rickettsia, strain MC16, isolated from ticks collected in Morocco. This organism shows a typical rickettsial morphology when analyzed by electron microscopy. After characterization by serotyping, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western immunoblotting, PCR-restriction fragment length polymorphism (RFLP), pulsed-field gel electrophoresis, and 16S rDNA sequencing, this organism was found to be different from all of the recognized SFG rickettsiae. Identical PCR-RFLP profiles have, however, been found in from Portugal and from Zimbabwe, which suggests that the distribution of this rickettsia reaches from the Mediterranean to southern Africa.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-47-2-548
1997-01-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/47/2/ijs-47-2-548.html?itemId=/content/journal/ijsem/10.1099/00207713-47-2-548&mimeType=html&fmt=ahah

References

  1. Babalis T., Tselentis Y., Roux V., Psaroulaki A., Raoult D. 1994; Isolation and identification of a rickettsial strain related to Rickettsia massiliae in Greek ticks. Am. J. Trop. Med. Hyg 50:365–372
    [Google Scholar]
  2. Bacellar F. Personal communication
  3. Bacellar F., Regnery L. R., Nuncio M. S., Filipe A. R. 1995; Genotypic evaluation of rickettsial isolates recovered from various species of ticks in Portugal. Epidemiol. Infect 114:169–178
    [Google Scholar]
  4. Bailly-Choumara H., Morel P. C., Rageau J. 1989; Première contribution au catalogue des tiques du Maroc (Acari, Ixodoidea). Bull. Soc. Sci. Phys. Nat. Maroc 54:71–80
    [Google Scholar]
  5. Beati L., Finidori J.-P., Gilot B., Raoult D. 1992; Comparison of serologic typing, sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein analysis, and genetic restriction fragment length polymorphism analysis for identification of rickettsiae: characterization of two new rickettsial strains. J. Clin. Microbiol 30:1922–1930
    [Google Scholar]
  6. Beati L., Finidori J.-P., Raoult D. 1993; First isolation of Rickettsia slovaca from Dermacentor marginatus in France. Am. J. Trop. Med. Hyg 48:257–268
    [Google Scholar]
  7. Beati L., Humair P.-F., Aeschlimann A., Raoult D. 1994; Identification of spotted fever group rickettsiae isolated from Dermacentor marginatus and Ixodes ricinus ticks collected in Switzerland. Am. J. Trop. Med. Hyg 51: ISSUS
    [Google Scholar]
  8. Beati L., Kelly P. J., Matthewman L. A., Mason P. R., Raoult D. 1995; Prevalence of rickettsia-like organisms and spotted fever group rickettsiae in ticks (Acari: Ixodidae) from Zimbabwe. J. Med. Entomol 32:140–154
    [Google Scholar]
  9. Beati L., Péter O., Burgdorfer W., Aeschlimann A., Raoult D. 1993; Confirmation that Rickettsia Helvetica sp. nov. is a distinct species of the spotted fever group of rickettsiae. Int. J. Syst. Bacteriol 43:521–526
    [Google Scholar]
  10. Beati L., Raoult D. 1993; Rickettsia massiliae sp. nov., a new spotted fever group rickettsia. Int. J. Syst. Bacteriol 43:839–840
    [Google Scholar]
  11. Beati L., Roux V., Ortuno A., Castella J., Segura Porta F., Raoult D. 1996; Phenotypic and genotypic characterization of spotted fever group rickettsiae isolated from Catalan Rhipicephalus sanguineus ticks. J. Clin. Microbiol 34:2688–2694
    [Google Scholar]
  12. Blanc G., Caminopteros J. 1932; Etudes épidémiologiques et expérimentales sur la fièvre boutonneuse, faites à l’institut Pasteur d’Athènes. Arch. Inst. Pasteur Tunis 20:343–394
    [Google Scholar]
  13. Burgdorfer W. 1970; Hemolymph test. A technique for detection of rickettsiae in ticks. Am. J. Trop. Med. Hyg 19:1010–1014
    [Google Scholar]
  14. Conor A., Bruch A. 1910; Une fièvre éruptive observée en Tunisie. Bull. Soc. Pathol. Exot 8:492–496
    [Google Scholar]
  15. Dessen P., Fondrat C., Valencien C., Munier G. 1990; BISANCE: a French service for access to biomolecular sequence databases. CABIOS 6:355–356
    [Google Scholar]
  16. Eremeeva M., Yu X., Raoult D. 1994; Differentiation among spotted fever group rickettsiae species by analysis of restriction fragment length polymorphism of PCR-amplified DNA. J. Clin. Microbiol 32:803–810
    [Google Scholar]
  17. Eremeeva M. E., Balayeva N. M., Raoult D. 1994; Purification of rickettsial cultures contaminated by mycoplasmas. Acta Virol 38:231–233
    [Google Scholar]
  18. Eremeeva M. E., Beati L., Makarova V. A., Fetisova N. F., Tarasevich I. V., Balayeva N. M., Raoult D. 1994; Astrakhan fever rickettsiae: antigenic and genotypic analysis of isolates obtained from human and Rhipicephalus pumilio ticks. Am. J. Trop. Med. Hyg 51:697–706
    [Google Scholar]
  19. Felsenstein J. 1989; PHYLIP—phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  20. Fox G. E., Wisotzkey J. D., Jurtshuk P. Jr. 1992; How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. Int. J. Syst. Bacteriol 42:166–170
    [Google Scholar]
  21. Giménez D. F. 1964; Staining rickettsiae in yolk-sac cultures. Stain Technol 39:135–140
    [Google Scholar]
  22. Giroud P., Colas-Belcour J., Pfister R., Morel P. 1957; Amblyomma, Hyalomma, Boophilus, rhipicephalus d’Afrique sont porteurs d’éléments rickettsiens et néorickettsiens et quelquefois des deux types d’agents. Bull. Soc. Pathol. Exot 50:529–532
    [Google Scholar]
  23. Heisch R. B., Grainger W. E., Harwey A. E., Lister G. 1962; Ferai aspects of rickettsial infections in Kenya. Trans. R. Soc. Trop. Med. Hyg 56:272–286
    [Google Scholar]
  24. Hoogstraal H. 1956 African Ixodoidea. I. Ticks of the Sudan (with special reference to Equatoria Province and with preliminary reviews of the genera Boophilus, Margaropus, and Hyalomma). Bureau of Medicine and Surgery, Department of the U.S. Navy; Washington, D.C.:
    [Google Scholar]
  25. Jukes T. H., Cantor C. R. 1969 Evolution of protein molecules. 21–132 Munro H. N.ed Mammalian protein metabolism Academic Press, Inc.; New York, N.Y.:
    [Google Scholar]
  26. Kelly P. J., Beati L., Mason P. R., Matthewman L. A., Roux V., Raoult D. 1996; Rickettsia africae sp. nov., the etiological agent of African tick bite fever. Int. J. Syst. Bacteriol 46:611–614
    [Google Scholar]
  27. Kelly P. J., Beati L., Matthewman L. A., Mason P. R., Raoult D. 1994; A new pathogenic spotted fever group rickettsia from Africa. J. Trop. Med. Hyg 97:129–137
    [Google Scholar]
  28. Kelly P. J., Mason P. R. 1990; Serological typing of spotted fever group rickettsia isolates from Zimbabwe. J. Clin. Microbiol 28:2302–2304
    [Google Scholar]
  29. Kelly P. J., Raoult D., Mason P. R. 1991; Isolation of spotted fever group rickettsias from triturated ticks using a modification of the centrifugation-shell vial technique. Trans. R. Soc. Trop. Med. Hyg 85:397–398
    [Google Scholar]
  30. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  31. Lange J. V., El Dessouky A. G., Manor E., Merdan A. I., Azad A. F. 1992; Spotted fever rickettsiae in ticks from the Northern Sinai Governate, Egypt. Am. J. Trop. Med. Hyg 46:546–551
    [Google Scholar]
  32. Meskini M., Beati L., Benslimane A., Raoult D. 1995; Seroepidemiology of rickettsial infections in Morocco. Eur. J. Epidemiol 11:655–660
    [Google Scholar]
  33. Ouhelli H., Pandey V. S., Benzaoui T. 1985; Seasonal variation of cattle ticks in a subhumid area of Morocco. Bull. Anim. Health Prod. Afr 33:207–210
    [Google Scholar]
  34. Péter O., Raoult D., Gilot B. 1990; Isolation by a sensitive centrifugation cell culture system of 52 strains of spotted fever group rickettsiae from ticks collected in France. J. Clin. Microbiol 28:1597–1599
    [Google Scholar]
  35. Philip R. N., Casper E. A., Burgdorfer W., Gerloff R. K., Hughes L. E., Bell E. J. 1978; Serologic typing of rickettsiae of the spotted fever group by indirect microimmunofluorescence. J. Immunol 121:1961–1968
    [Google Scholar]
  36. Regnery R. L., Spruill C. L., Plikaytis B. D. 1991; Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J. Bacteriol 173:1576–1589
    [Google Scholar]
  37. Roux V., Raoult D. 1993; Genotypic identification and phylogenetic analysis of the spotted fever group rickettsiae by pulsed-field gel electrophoresis. J. Bacteriol 175:4895–4904
    [Google Scholar]
  38. Roux V., Raoult D. 1995; Phylogenetic analysis of the genus Rickettsia by 16S rDNA sequencing. Res. Microbiol 146:385–396
    [Google Scholar]
  39. Roux V., Rydkina E., Eremeeva M., Raoult D. Citrate synthase gene comparison, a new tool for phylogenetical analysis and its application for the rickettsiae. Submitted for publication
  40. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol 4:406–425
    [Google Scholar]
  41. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol 44:846–849
    [Google Scholar]
  42. Tissot Dupont H., Cornet J.-P., Raoult D. 1994; Identification of rickettsiae from ticks collected in the Central African Republic using the polymerase chain reaction. Am. J. Trop. Med. Hyg 50:373–380
    [Google Scholar]
  43. Towbin A., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350–4354
    [Google Scholar]
  44. Walker D. H. 1989; Rocky Mountain spotted fever: a disease in need of microbiological concern. Clin. Microbiol. Rev 2:227–240
    [Google Scholar]
  45. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. R., Moore L. H., Moore W. E. C., Murray R. G. E., Stackebrandt E., Starr M. P., Triiper H. G. 1987; Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics. Int. J. Syst. Bacteriol 37:463–464
    [Google Scholar]
  46. Weiss E., Coolbaugh J. C., Williams J. C. 1975; Separation of viable Rickettsia typhi from yolk sac and L cell host components by Renografin density gradient centrifugation. Appl. Microbiol 30:456–463
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-47-2-548
Loading
/content/journal/ijsem/10.1099/00207713-47-2-548
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error