1887

Abstract

Human astroviruses are an important cause of gastroenteritis. As part of a molecular epidemiological study carried out in Mexico a human astrovirus isolate, Yuc-8, was adapted to grow in CaCo-2 cells, and its entire genome was sequenced. A 15 amino acid deletion in ORF1a, which has been associated with adaptation of astroviruses to grow in cells other than CaCo-2, was present in Yuc-8. Comparative sequence analysis of the Yuc-8 ORF2 with reported human astrovirus sequences revealed that this isolate belongs to genotype (serotype) 8. Two distinct domains in ORF2 were observed: an amino-terminal domain (residues 1 to 415), with identities higher than 81% among the strains analysed, and a carboxy-terminal domain (residues 416 to 782) with identities between 36 and 60%. Two non-superimposable phylogenetic trees were generated by separate analysis of these two domains, suggesting that a differential selective pressure is exerted along the structural polyprotein.

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2000-12-01
2024-03-28
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References

  1. Bass D., Qiu S. 2000; Proteolytic processing of the astrovirus capsid. Journal of Virology 74:1810–1814
    [Google Scholar]
  2. Bass D., Upadhyayula U. 1997; Characterization of human serotype 1 astrovirus-neutralizing epitopes. Journal of Virology 71:8666–8671
    [Google Scholar]
  3. Belliot G., Laveran H., Monroe S. 1997; Detection and genetic differentiation of human astroviruses: phylogenetic grouping varies by coding region. Archives of Virology 142:1323–1334
    [Google Scholar]
  4. Gaggero A., O’Ryan M., Noel J., Glass R., Monroe S., Mamani N., Prado V., Avendano L. 1998; Prevalence of astrovirus infection among Chilean children with acute gastroenteritis. Journal of Clinical Microbiology 36:3691–3693
    [Google Scholar]
  5. Herrmann J., Hudson R., Perron-Henry D., Kurtz J., Blacklow N. 1988; Antigenic characterization of cell-cultivated astrovirus serotypes and development of astrovirus-specific monoclonal antibodies. Journal of Infectious Diseases 158:182–185
    [Google Scholar]
  6. Herrmann J., Cubitt W., Hudson R., Perron-Henry D., Oshiro L., Blacklow N. 1990; Immunological characterization of the Marin County strain of astrovirus. Archives of Virology 110:213–220
    [Google Scholar]
  7. Herrmann J., Taylor D., Echeverria P., Blacklow N. 1991; Astroviruses as a cause of gastroenteritis in children. New England Journal of Medicine 324:1757–1760
    [Google Scholar]
  8. Jiang B., Monroe S., Koonin E., Stine S., Glass R. 1993; RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frameshifting signal that directs the viral replicase synthesis. Proceedings of the National Academy of Sciences, USA 90:10539–10543
    [Google Scholar]
  9. Jonassen C., Jonassen T., Grinde B. 1998; A common RNA motif in the 3′ end of the genomes of astroviruses, avian infectious bronchitis virus and an equine rhinovirus. Journal of General Virology 79:715–718
    [Google Scholar]
  10. Lewis T., Matsui S. 1997; Studies of the astrovirus signal that induces (−1) ribosomal frameshifting. Advances in Experimental Medicine Biology 412:323–330
    [Google Scholar]
  11. Lopez S., Arias C., Mendez E., Espejo R. 1986; Conservation in rotaviruses of the protein region containing the two sites associated with trypsin enhancement of infectivity. Virology 154:224–7
    [Google Scholar]
  12. Marczinke B., Bloys A., Brown T., Willcocks M., Carter M., Brierley I. 1994; The human astrovirus RNA-dependent RNA polymerase coding region is expressed by ribosomal frameshifting. Journal of Virology 68:5588–5595
    [Google Scholar]
  13. Monceyron C., Grinde B., Jonassen T. 1997; Molecular characterization of the 3′-end of the astrovirus genome. Archives of Virology 142:699–706
    [Google Scholar]
  14. Monroe S., Stine S., Gorelkin L., Herrmann J., Blacklow N., Glass R. 1991; Temporal synthesis of proteins and RNAs during human astrovirus infection of cultured cells. Journal of Virology 65:641–648
    [Google Scholar]
  15. Monroe S., Jiang B., Stine S., Koopmans M., Glass R. 1993; Subgenomic RNA sequence of human astrovirus supports classification of Astroviridae as a new family of RNA viruses. Journal of Virology 67:3611–3614
    [Google Scholar]
  16. Mustafa H., Palombo E., Bishop R. 2000; Epidemiology of astrovirus Infection in young children hospitalized with acute gastroenteritis in Melbourne, Australia, over a period of four consecutive years, 1995 to 1998. Journal of Clinical Microbiology 38:1058–1062
    [Google Scholar]
  17. Noel J., Cubitt D. 1994; Identification of astrovirus serotypes from children treated at the Hospitals for Sick Children, London 1981–93. Epidemiology and Infection 113:153–159
    [Google Scholar]
  18. Noel J., Lee T., Kurtz J., Glass R., Monroe S. 1995; Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing. Journal of Clinical Microbiology 33:797–801
    [Google Scholar]
  19. Palombo E., Bishop R. 1996; Annual incidence, serotype distribution, and genetic diversity of human astrovirus isolates from hospitalized children in Melbourne, Australia. Journal of Clinical Microbiology 34:1750–1753
    [Google Scholar]
  20. Saito K., Ushijima H., Nishio O., Oseto M., Motohiro H., Ueda Y., Takagi M., Nakaya S., Ando T., Glass R. and others 1995; Detection of astroviruses from stool samples in Japan using reverse transcription and polymerase chain reaction amplification. Microbiology and Immunology 39:825–828
    [Google Scholar]
  21. Sanchez-Fauquier A., Carrascosa A., Carrascosa J., Otero A., Glass R., Lopez J., Martin C. S., Melero J. 1994; Characterization of a human astrovirus serotype 2 structural protein (VP26) that contains an epitope involved in virus neutralization. Virology 201:312–320
    [Google Scholar]
  22. Shastri S., Doane A., Gonzales J., Upadhyayula U., Bass D. 1998; Prevalence of astroviruses in a children’s hospital. Journal of Clinical Microbiology 36:2571–2574
    [Google Scholar]
  23. Willcocks M., Ashton N., Kurtz J., Cubitt W., Carter M. 1994a; Cell culture adaptation of astrovirus involves a deletion. Journal of Virology 68:6057–6058
    [Google Scholar]
  24. Willcocks M., Brown T., Madeley C., Carter M. 1994b; The complete sequence of a human astrovirus. Journal of General Virology 75:1785–1788
    [Google Scholar]
  25. Willcocks M., Kurtz J., Lee T. W., Carter M. 1995; Prevalence of human astrovirus serotype 4: capsid protein sequence and comparison with other strains. Epidemiology and infection 114:385–394
    [Google Scholar]
  26. Willcocks M., Boxall A., Carter M. 1999; Processing and intracellular location of human astrovirus non-structural proteins. Journal of General Virology 80:2607–2611
    [Google Scholar]
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