1887

Abstract

SUMMARY: The and genes are specifically expressed in the dikaryon of the basidiomycete fungus during fruiting. These genes are closely linked (within 6 kb) and highly similar in gene structure and nucleotide sequence (70% identical nucleotides in their coding regions). The encoded proteins (204 and 214 amino acids, respectively) have 87% similarity in amino acids (56% of the amino acids are identical). They contain putative signal sequences for secretion, are rich in aromatic amino acids which are generally located at similar positions, and they are generally hydrophilic. Inspection of databanks showed similarities with pathogenesis-related proteins (PR1) from plants, testis-specific proteins from mammals and venom allergen proteins from insects. An antibody raised against a Sc7 fusion protein showed the presence of the Sc7 protein in the culture medium and in the fruit bodies where it is apparently loosely associated with hyphal walls.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-139-9-2083
1993-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/139/9/mic-139-9-2083.html?itemId=/content/journal/micro/10.1099/00221287-139-9-2083&mimeType=html&fmt=ahah

References

  1. Benoist C., O’Fiare K., Breathnach R. 1980; The ovalbumin gene: sequence of putative control regions.. Nucleic Acids Research 8:127–142
    [Google Scholar]
  2. Brooks D.E., Means A.R., Wright E.J., Singh S.P., Tiver K.K. 1986; Molecular cloning of the cDNA for androgen- dependent sperm-coating glycoproteins secreted by the rat epididymis.. European Journal of Biochemistry 161:13–18
    [Google Scholar]
  3. Cornelissen B.J.C., Hooft Van Huijsduunen R.A.M., Van Loon L.C., Bol J. 1986; Molecular characterization of messenger RNAs for ‘pathogenesis-related’ proteins la, lb and lc, induced by TMV infection of tobacco.. EMBO Journal 5:37–40.
    [Google Scholar]
  4. Dayhoff M.O. 1978; A model of evolutionary change in proteins. Matrices for detecting distant relationships.. In Atlas of Protein Sequence and Structure 5 suppl. 3 pp 345–358 Dayhoff M.O. Edited by Washington, DC: National Biomedical Research Foundation.;
    [Google Scholar]
  5. De Vries O.M.H., Fekkes M.P., Wösten H.A.B., Wessels J.G.H. 1993; Insoluble hydrophobin complexes in the walls of Schizophyllum commune and other filamentous fungi.. Archives of Microbiology 159:330–335
    [Google Scholar]
  6. Dons J.J.M., Devries O.M.H., Wessels J.G.H. 1979; Characterization of the genome of the basidiomycete Schizophyllum commune. . Biochimica et Biophysica Acta 563:100–112
    [Google Scholar]
  7. Fang K.S.Y., Vitale M., Fehlner P., King T.P. 1988; cDNA cloning and primary structure of a white-face hornet venom allergen, antigen 5.. Proceedings of the National Academy of Sciences of the United States of America 85:895–899
    [Google Scholar]
  8. Giasson L., Specht C.A., Milgrim C., Novotny C.P., Ullrich R.C. 1989; Cloning and comparison of Aa mating-type alleles of the basidiomycete Schizophyllum commune. . Molecular and General Genetics 218:72–77
    [Google Scholar]
  9. Gurr S.J., Unkles S.E., Kinghorn J. 1987; The structure and organization of nuclear genes in filamentous fungi.. In Gene Structure in Eukaryotic Microbes, SGM Special Publications 26 pp 93–139 Kinghorn J.R. Edited by Oxford: IRL Press.;
    [Google Scholar]
  10. Harlow E., Lane D. 1988 Antibodies: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  11. Higgins D.G., Sharp P. 1988; CLUSTAL: a package for performing multiple sequence alignment on a microcomputer.. Gene 73:237–244
    [Google Scholar]
  12. Kasahara M., Gutknecht J., Brew K., Spurr N., Goodfellow P.N. 1989; Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene.. Genomics 5:527–534
    [Google Scholar]
  13. Kozak M. 1984; Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.. Nucleic Acids Research 12:857–872
    [Google Scholar]
  14. Kyte J., Doolittle R.F. 1982; A simple method for displaying the hydrophobic character of a protein.. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  15. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature; London: 227680–685
    [Google Scholar]
  16. Lipman D.J., Pearson W.R. 1985; Rapid and sensitive protein similarity searches.. Science 227:1435–1441
    [Google Scholar]
  17. Lucas J., Camacho Henriquez A., Lottspeich F., Henschen A., Sänger H.L. 1985; Amino acid sequence of the ‘pathogenesis- related’ leaf protein pl4 from viroid-infected tomato reveals a new type of structurally unfamiliar proteins.. EMBO Journal 4:2745–2749
    [Google Scholar]
  18. Mulder G.H., Wessels J.G.H. 1986; Molecular cloning of RNAs differentially expressed in monokaryons and dikaryons ofSchizophyllum commune in relation to fruiting.. Experimental Mycology 10:214–227
    [Google Scholar]
  19. Ruiters M.H.J., Sietsma J.H., Wessels J.G.H. 1988; Expression of dikaryon-specific mRNAs ofSchizophyllum commune in relation to incompatibility genes, light, and fruiting.. Experimental Mycology 12:60–69
    [Google Scholar]
  20. Sambrook J., Fritsch E.F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual,, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain terminating inhibitors.. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  22. Schuren F.H.J., Wessels J.G.H. 1990; Two genes specifically expressed in fruiting dikaryons of Schizophylluna commune : homologies with a gene not regulated by mating-type genes.. Gene 90:199–205
    [Google Scholar]
  23. Specht C.A., Stankis M.M., Giasson L., Novotny C.P., Ullrich R.C. 1992; Functional analysis of the homeodomain- related proteins of theAa locus ofSchizophyllum commune. . Proceedings of the National Academy of Sciences of the United States of America 89:7174–7178
    [Google Scholar]
  24. Spiro R.G. 1973; Glycoproteins.. Advances in Protein Chemistry 27:349–367
    [Google Scholar]
  25. Springer J., Wessels J.G.H. 1989; A frequently occurring mutation that blocks the expression of fruiting genes inSchizophyllum commune. . Molecular and General Genetics 219:486–488
    [Google Scholar]
  26. Stankis M.M., Specht C.A., Yang H., Giasson L., Ullrich R.C., Novotny C.P. 1992; TheAα mating locus ofSchizophyllum commune encodes two dissimilar multiallelic homeodomain proteins.. Proceedings of the National Academy of Sciences of the United States of America 89:7169–7173
    [Google Scholar]
  27. Watson M.E.E. 1984; Compilation of published signal sequences.. Nucleic Acids Research 12:5145–5164
    [Google Scholar]
  28. Wessels J.G.H. 1992; Gene expression during fruiting inSchizophyllum commune. . Mycological Research 96:609–620
    [Google Scholar]
  29. Wessels J.G.H., De VRIES O.M.H., Ásgeirsdóttlr S.A., Springer J. 1991a; Thethn mutation ofSchizophyllum commune,which suppresses formation of aerial hyphae, affects expression of theSc3 hydrophobin gene.. Journal of General Microbiology 137:2439–2445
    [Google Scholar]
  30. Wessels J.G.H., De Vries O.M.H., Ásgeirsdóttlr S.A., Schuren F.H.J. 1991b; Hydrophobin genes involved in form-ation of aerial hyphae and fruit bodies inSchizophyllum. . Plant Cell 3:793–799
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-139-9-2083
Loading
/content/journal/micro/10.1099/00221287-139-9-2083
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error