1887

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Volume 97, Issue 9

Bluetongue virus serotype 27: detection and characterization of two novel variants in Corsica, France Free

Abstract

During the compulsory vaccination programme against bluetongue virus serotype 1 (BTV-1) in Corsica (France) in 2014, a BTV strain belonging to a previously uncharacterized serotype (BTV-27) was isolated from asymptomatic goats. The present study describes the detection and molecular characterization of two additional distinct BTV-27 variants found in goats in Corsica in 2014 and 2015. The full coding genome of these two novel BTV-27 variants show high homology (90–93 % nucleotide/93–95 % amino acid) with the originally described BTV-27 isolate from Corsican goats in 2014. These three variants constitute the novel serotype BTV-27 (‘BTV-27/FRA2014/v01 to v03’). Phylogenetic analyses with the 26 other established BTV serotypes revealed the closest relationship to BTV-25 (SWI2008/01) (80 % nucleotide/86 % amino acid) and to BTV-26 (KUW2010/02) (73–74 % nucleotide/80–81 % amino acid). However, highest sequence homologies between individual segments of BTV-27/FRA2014/v01–v03 with BTV-25 and BTV-26 vary. All three variants share the same segment 2 nucleotype with BTV-25. Neutralization assays of anti-BTV27/FRA2014/v01–v03 sera with a reassortant virus containing the outer capsid proteins of BTV-25 (BTV1) further confirmed that BTV-27 represents a distinct BTV serotype. Relationships between the variants and with BTV-25 and BTV-26, hypotheses about their origin, reassortment events and evolution are discussed.

  • Received:
  • Accepted:
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Keyword(s): Bluetongue , BTV serotype 27 , complete coding genome sequences , France and new variants
© 2016 The Authors
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2016-09-01
2024-04-25
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Bluetongue virus serotype 27: detection and characterization of two novel variants in Corsica, France
J. Gen. Virol. 97, 2073 (2016); https://doi.org/10.1099/jgv.0.000557
/content/journal/jgv/10.1099/jgv.0.000557
/content/journal/jgv/10.1099/jgv.0.000557
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