1887

Abstract

The purpose of the present study was to analyse mutations in the and genes of and define the possible correlation between these mutations and resistance to levofloxacin (LVX), moxifloxacin (MFX) and gatifloxacin (GAT), based on their MICs. One hundred and forty-two clinical isolates were collected from pulmonary tuberculosis patients in the Moscow region. All strains were tested for drug susceptibility to rifampicin and isoniazid using the BACTEC MGIT 960 System and to ofloxacin (OFX) using the absolute concentration method on solid Lowenstein–Jensen slants. All in all, 68 strains were selected at random (38 strains were resistant and 30 were susceptible to OFX) for further analysis using the TB-BIOCHIP-2 test system and DNA sequence analysis. The MICs of LVX, MFX and GAT for selected strains were determined using the BACTEC MGIT 960 System. Mutations in the gene were observed in 36 out of 38 (94.7 %) OFX-resistant strains. Asn538Asp and Asp500His substitutions in the gene only were found in two (5.3 %) strains. Twenty-nine out of 30 OFX-sensitive strains had no mutations in either gene. One (3.3 %) OFX-sensitive strain carried an Arg485His substitution in . The results of our investigation showed that there is no clear correlation between the type of mutation in the genes and , and the MIC levels of LVX, MFX and GAT for resistant strains. Mutations in and Asn538Asp, and Asp500His substitutions in were associated with cross-resistance of to fluoroquinolones. The substitution Arg485His in does not confer resistance to LVX, MFX and GAT in .

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2013-01-01
2024-03-29
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