1887

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Volume 94, Issue 3

Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement Free

Abstract

(FMV), a member of the newly formed genus , is a segmented negative-strand RNA virus. Each of the six genomic FMV segments contains a single ORF: that of RNA4 encodes the protein p4. FMV-p4 is presumed to be the movement protein (MP) of the virus; however, direct experimental evidence for this is lacking. We assessed the intercellular distribution of FMV-p4 in plant cells by confocal laser scanning microscopy and we found that FMV-p4 was localized to plasmodesmata and to the plasma membrane accompanied by tubule-like structures. A series of experiments designed to examine the movement functions revealed that FMV-p4 has the capacity to complement viral cell-to-cell movement, prompt GFP diffusion between cells, and spread by itself to neighbouring cells. Altogether, our findings demonstrated that FMV-p4 shares several properties with other viral MPs and plays an important role in cell-to-cell movement.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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2013-03-01
2024-04-19
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Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement
J. Gen. Virol. 94, 682 (2013); https://doi.org/10.1099/vir.0.047860-0
/content/journal/jgv/10.1099/vir.0.047860-0
/content/journal/jgv/10.1099/vir.0.047860-0
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