Let-7 microRNA-mediated mRNA deadenylation and translational repression in a mammalian cell-free system
- 1 RIKEN Genomic Sciences Center, Protein Research Group, Tsurumi, Yokohama 230-0045, Japan;
- 2 Kazusa DNA Research Institute, Department of Human Genome Research, Kisarazu, Chiba 292-0818, Japan;
- 3 RIKEN Research Center for Allergy and Immunology, Laboratory for Immunogenomics, Tsurumi, Yokohama 230-0045, Japan;
- 4 The University of Tokyo, Graduate School of Science, Bunkyo, Tokyo 113-0033, Japan
Abstract
MicroRNAs (miRNAs) are incorporated into miRNP complexes and regulate protein expression post-transcriptionally through binding to 3′-untranslated regions of target mRNAs. Here we describe a recapitulation of let-7 miRNA-mediated translational repression in a cell-free system, which was established with extracts prepared from HEK293F cells overexpressing miRNA pathway components. In this system, both the cap and poly(A) tail are required for the translational repression, and let-7 directs the deadenylation of target mRNAs. Our work suggests that let-7 miRNPs containing Argonaute and GW182 impair the synergistic enhancement of translation by the 5′-cap and 3′-poly(A) tail, resulting in translational repression.
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Footnotes
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↵5 Corresponding author.
↵5 E-MAIL yokoyama{at}biochem.s.u-tokyo.ac.jp; FAX 81-45-503-9195.
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Supplemental material is available at http://www.genesdev.org.
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Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.1566707
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- Received May 1, 2007.
- Accepted June 22, 2007.
- Copyright © 2007, Cold Spring Harbor Laboratory Press
