Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.

N Baskaran; R P Kandpal; A K Bhargava; M W Glynn; A Bale; S M Weissman

doi:10.1101/gr.6.7.633

Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.

N Baskaran,
R P Kandpal,
A K Bhargava,
M W Glynn,
A Bale, and
S M Weissman

Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA.

Abstract

A PCR method for uniform amplification of a mixture of DNA templates differing in GC content is described using the two enzyme approach (Klentaq1 and Pfu DNA polymerase) and a combination of DMSO and betaine. This method was applied to amplify the CGG repeat region from the fragile X region.