Heterogeneity in endothelial cells from large vessels and microvessels

doi:10.1111/j.1432-0436.1987.tb00181.x

Differentiation

Volume 36, Issue 1, November 1987, Pages 57-70

https://doi.org/10.1111/j.1432-0436.1987.tb00181.x Get rights and content

Abstract

The successful isolation and culture of vascular endothelial cells has led to an upsurge of interest in their role in such diverse processes as thrombogenesis, atherosclerosis and tumour growth. In this article we have outlined methods for the culture and characterization of endothelial cells from large vessels, capillary and post-capillary venules of lymph nodes. Comparison of their immunological and metabolic properties illustrates the heterogeneity within the vasculature. The effect of growth and angiogenic factors on these cells and the efficacy of their use in culture medium is considered.

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Heterogeneity in endothelial cells from large vessels and microvessels

Abstract

Biochem Biophys Res Commun

Lancet

Atherosclerosis

J Atheroscler Res

Microvasc Res

Vision Res

Am Heart J

Lancet

Blood

J Ultrastruct Res

Microvasc Res

Anal Biochem

Isolation and culture of high endothelial cells from rat lymph nodes

J Cell Sci

Microvascular changes in lymph nodes draining skin allografts

Am J Pathol

Specialised structure and metabolic activities of high endothelial venules in rat lymphatic tissues

Immunology

Migration of lymphocytes across specialised vascular endothelium V. Production of a sulphated macromolecule by high endothelial cells in lymph nodes

J Cell Sci

Glyconjugates from high endothelial cells I. Partial characterisation of a sulphated glycoconjugate from the high endothelial cells of rat lymph nodes

J Cell Sci

The development of peripheral blood vessels

Intimal ultrastructure of human umbilical arteries

Circ Res

Differential angiogenesis, mechanisms and pathobiology

Cell surface markers on endothelial cells: a developmental perspective

Identification and partial characterisation of two major proteins of Mr 47 000 synthesised by bovine retinal endothelial cells

J Biochem

Microvascular endothelium and pericytes: high yield, low passage cultures

In Vitro Cell Dev Biol

Lymphocyte recognition of lymph node endothelium. IV. Cell surface structures mediating entry into lymph nodes

J Immunol

Biological structure and function, no. 6

Long term cultures of capillary endothelial cells

Proc Natl Acad Sci USA

Lymphocyte migration and immune responses

Prog Allergy

The preparation and handling of lymphocytes

Differences in the uptake of modified low density lipoproteins by tissue cultured endothelial cells

J Cell Sci

A cell-surface molecule involved in organ-specific homing of lymphocytes

Nature

Human vascular endothelial cells in culture

J Cell Biol

Long-term serial cultivation of arterial and capillary endothelium from adult bovine brain

In Vitro Cell Dev Biol

The route of recirculation of lymphocytes in the rat

Proc R Soc Lond [Biol]

Disappearance and reappearance of high endothelial venules and immigrating lymphocytes in lymph nodes deprived of afferent lymphatic vessels

Eur J Immunol

Endothelial growth factors and extracellular matrix regulate DNA synthesis through modulation of cell and nuclear expansion

In Vitro Cell Dev Biol

Purification and characterization of rat bone marrow endothelial cells

Exp Hematol

Culture and identification of large vessel endothelial cells

Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria

J Clin Invest

Identification and partial characterisation of two major proteins of M_r 47 000 synthesised by bovine retinal endothelial cells