AnaeroPack^® Malaria Culture System (SUGIYAMA-GEN Co., Ltd.) using AnaeroPack^®·plas (5%O₂, 5%CO₂) and AnaeroPack^®·CO₂ (15%O₂, 6%CO2) was evaluated by comparing with the standard laboratory in vitro continuous culture technique. Two culture-adapted strains of Plasmodium falciparum, SGE-1 (chloroquine sensitive strain) and K1 (chloroquine resistant strain), were continuously cultured for 26 days in vitro under the 3 systems. The parasite proliferation curves under the different set systems were paralleled in both strains, which demonstrate that this AnaeroPack^® Malaria Culture System is useful for the culture-adapted strains of P. falciparum. Although further test using isolates from falciparum malaria patients should be carried out, the AnaeroPack^® Malaria Culture System seems promising for the culture in the field studies.