In vitro study of the Photodynamic Antimicrobial Therapy (PACT) against promastigotes form of the Leishmania (Viannia) braziliensis: In Vitro Study

Artur F. S. Barbosa; Bruno B. Sangiorgi; Suely L. Galdino; Ivan R. Pitta; Manoel Barral-Netto; Antônio L. B. Pinheiro

doi:10.1117/12.2005157

5 March 2013 In vitro study of the photodynamic antimicrobial therapy (PACT) against promastigotes form of the leishmania (viannia) braziliensis: in vitro study

Artur F. S. Barbosa, Bruno B. Sangiorgi, Suely L. Galdino, Ivan R. Pitta, Manoel Barral-Netto, Antônio L. B. Pinheiro

Author Affiliations +

Proceedings Volume 8569, Mechanisms for Low-Light Therapy VIII; 85690N (2013) https://doi.org/10.1117/12.2005157
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Leishmaniasis, a protozoan parasitic disease that remains a major worldwide health problem with high endemicity in developing countries. Treatment of cutaneous Leishmaniasis (CL) should be decided by the clinical lesions, etiological species and its potential to develop into mucosal Leishmaniasis. High cost, systemic toxicity, and diminished efficacy due to development of parasite resistance are the serious drawbacks of current treatment options. Thus, identifying new, effective, and safer anti-leishmanial drug(s) is of paramount importance. The aim of this study was to verify the effectiveness of PACT in vitro, as a new technique for the treatment of Leishmaniasis. For this, semiconductor laser (λ = 660nm, 40mW, 8.4J/cm², CW) associated to phenothiazine’s derivatives (5 and 10 μg/ml, TBO, Methylene Blue or Phenothiazine) on the promastigotes form of Leishmania braziliensis in a single session was used. Viability of the parasites was assessed in quadruplicates of each group. The samples were removed and analyzed in a hemocytometer 72h after PACT. We found an important decrease in the number of viable parasites on all treated groups in comparison to their controls. The results of present study showed significant percentage of lethality (above 92%) of the protocol. The 98.33% of lethality was achieved with 10 μg/ml of FTZ. No lethality was seen on groups treated neither with laser nor with each compounds separately. The results are promising and indicative that the use of PACT may be a powerful treatment of Leishmaniasis when compared to already available ones.

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Artur F. S. Barbosa, Bruno B. Sangiorgi, Suely L. Galdino, Ivan R. Pitta, Manoel Barral-Netto, and Antônio L. B. Pinheiro "In vitro study of the photodynamic antimicrobial therapy (PACT) against promastigotes form of the leishmania (viannia) braziliensis: in vitro study", Proc. SPIE 8569, Mechanisms for Low-Light Therapy VIII, 85690N (5 March 2013); https://doi.org/10.1117/12.2005157

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