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Glycobiology of human milk

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Abstract

Glycans are characteristic components of milk, and each species has unique patterns of specific carbohydrates. Human milk is unusually rich in glycans, with the major components being lactose and oligosaccharides, representing approximately 6.8 and 1% of the milk, respectively. Other sources of glycans in human milk include monosaccharides, mucins, glycosaminoglycans, glycoproteins, glycopeptides, and glycolipids. In human milk, the presence and patterns of these glycans vary depending upon the stage of lactation and the maternal genes and their genetic polymorphisms that control glycosyl transferases. The synthesis of milk glycans utilizes a significant portion of the metabolic energy that the mother expends when producing her milk, but other than lactose, these glycans contribute little to the nutritional needs of the infant. The data herein support several functions. 1) Many human milk glycans inhibit pathogens from binding to the intestinal mucosa. 2) Human milk glycans attenuate inflammation. 3) Glycans also directly stimulate the growth of beneficial (mutualist) bacteria of the microbiota (formerly considered commensal microflora of the intestine); these mutualists and their fermentation products can, in turn, (a) inhibit pathogens, (b) modulate signaling and inflammation, and (c) the fermentation products can be absorbed and utilized as a source of dietary calories. These functions can help direct and support intestinal postnatal growth, development, and ontogeny of colonization. The many functions of the milk glycans may synergistically protect infants from disease. Hence, human milk glycans and their homologs may serve as novel prophylactic or therapeutic agents for a diverse range of deleterious conditions.

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Abbreviations

GAG:

glycosaminoglycan

HMOS:

human milk oligosaccharides

LDFH-I:

lactodifucohexaose-1

LDFT:

lactodifucotetraose

Neu5Ac:

N-acetylneuraminic acid

Neu5Gc:

N-glycolylneuraminic acid

2′-FL:

2′-fucosyllactose

3-FL:

3-fucosyllactose

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  1. Department of Biology, Program in Glycobiology, 140 Commonwealth Avenue, Boston College, Chestnut Hill, MA, 02467, USA

    D. S. Newburg

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Correspondence to D. S. Newburg.

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Dedicated to my mentor and friend, Dr. Robert H. McCluer (April 13, 1928–September 8, 2005)

Published in Russian in Biokhimiya, 2013, Vol. 78, No. 7, pp. 990–1007.

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Newburg, D.S. Glycobiology of human milk. Biochemistry Moscow 78, 771–785 (2013). https://doi.org/10.1134/S0006297913070092

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