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Low-level laser therapy (LLLT) reduces inflammatory infiltrate and enhances skeletal muscle repair: Histomorphometric parameters

  • Laser Methods in Chemistry, Biology, and Medicine
  • Published:
Laser Physics

Abstract

Low level laser therapy (LLLT) has been suggested as an effective therapeutics in inflammatory processes modulation and tissue repairing. However, there is a lack of studies that analyze the anti-inflammatory effects of the infrared lasers in muscular skeletal injury. The aim of this study was to investigate the effects of low-level laser therapy 904 nm in the repair process of skeletal muscle tissue. Swiss mice were submitted to cryoinjury and divided in test (LLLT-treated) and control groups. Histological sections were stained with hematoxylin-eosin to assess general morphology and inflammatory influx, and Picrossirus to quantify collagen fibers deposition. Our results showed significant reduction in inflammatory infiltrated in irradiated mice after 4 days of treatment compared to control (p = 0.01). After 8 days, the irradiated group showed high levels at regenerating myofibers with significant statistically differences in relation at control group (p < 0.01). Collagen deposition was significantly increased in the final stages of regeneration at test group, when compared with control group (p = 0.05). Our data suggests that LLLT reduces the inflammatory response in the initial stages of injury and accelerates the process of muscular tissue repair.

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Authors and Affiliations

  1. Department of Immunobiology, Institute of Biology, Neuroscience Master Science Program, Fluminense Federal University (UFF), Niterói, RJ, Brazil

    E. L. Paiva-Oliveira

  2. Physiotherapy Course of Faculty of Minas (FAMINAS), Muriaé, MG, Brazil

    N. C. Lima, P. H. Silva & N. T. A. Sousa

  3. Department of Physiotherapy, Nilton Lins University Center (UNINILTONLINS), Manaus, AM, Brazil

    F. S. Barbosa

  4. Department of Physiotherapy, Analysis of Human Movement Lab, Science of Rehabilitation Program, Augusto Motta University Center (UNISUAM), Rio de Janeiro, RJ, Brazil

    M. Orsini & J. G. Silva

  5. Department of Physiotherapy, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil

    J. G. Silva

Authors
  1. E. L. Paiva-Oliveira
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  2. N. C. Lima
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  3. P. H. Silva
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  4. N. T. A. Sousa
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  5. F. S. Barbosa
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  6. M. Orsini
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  7. J. G. Silva
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Correspondence to E. L. Paiva-Oliveira.

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Original Text © Astro, Ltd., 2012.

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Paiva-Oliveira, E.L., Lima, N.C., Silva, P.H. et al. Low-level laser therapy (LLLT) reduces inflammatory infiltrate and enhances skeletal muscle repair: Histomorphometric parameters. Laser Phys. 22, 1425–1430 (2012). https://doi.org/10.1134/S1054660X12090113

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  • DOI: https://doi.org/10.1134/S1054660X12090113

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