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Study of DNA import and export in potato (Solanum tuberosum) mitochondria using quantitative PCR

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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology Aims and scope

Abstract

To study DNA transport in isolated mitochondria from potato tubers (Solanum tuberosum), we developed an approach permitting the analysis of internalized DNA by quantitative PCR. It was shown that mitochondrial transport of recombinant plasmid pBluescript IIKS (+) of the size of 3050 bp and DNA fragment of 89 bp amplified from human LINE1 repeat did take place, apparently through different membrane translocation mechanisms. Regardless of the length of the polynucleotide chain, DNA transport into mitochondria was a reversible process.

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References

  1. Turmel M., Otis C., Lemieux C. 2002. The complete mitochondrial DNA sequence of Mesostigma viride identifies this green alga as the earliest green plant divergence and predicts a highly compact mitochondrial genome in the ancestor of all green plants. Mol. Biol. Evol. 19, 24–38.

    PubMed  CAS  Google Scholar 

  2. Kubo T., Nishizawa S., Sugawara A., Itchoda N., Estiati A., Mikami T. 2000. The complete nucleotide sequence of the mitochondrial genome of sugar beet (Beta vulgaris L.) reveals a novel gene for tRNA(Cys)(GCA). Nucl. Acids Res. 28, 2571–2576.

    Article  PubMed  CAS  Google Scholar 

  3. Marienfeld J., Unseld M., Brennicke A. 1999. The mitochondrial genome of Arabidopsis is composed of both native and immigrant information. Trends Plant Sci. 4, 495–502.

    Article  PubMed  Google Scholar 

  4. Unseld M., Marienfeld J.R., Brandt P., Brennicke A. 1997. The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366.924 nucleotides. Nat. Genet. 15, 57–61.

    Article  PubMed  CAS  Google Scholar 

  5. Koulintchenko M., Konstantinov Y., Dietrich A. 2003. Plant mitochondria actively import DNA via the permeability transition pore complex. EMBO J. 22, 1245–1254.

    Article  PubMed  CAS  Google Scholar 

  6. Konstantinov Y.M., Podsosonny V.A., Lutsenko G.N., Rivkin M.I. 1989. Translocation of bacterial vector plazmids into mitochondria of maize seedlings. Biochemistry (Rus.). 54(1), 154–157.

    CAS  Google Scholar 

  7. Atchison B.A., Devenish R.J., Linnane A.W., Nagley R. 1980. Transformation of Saccharomyces cerevisiae with yeast mitochondrial DNA linked to two micron circular yeast plasmid. Biochem. Biophys. Res. Commun. 96, 580–586.

    Article  PubMed  CAS  Google Scholar 

  8. Bej A.K., Perlin M.H. 1991. Acquisition of mitochondrial DNA by a transformation vector for Ustilago violacea. Gene. 98, 135–40.

    Article  PubMed  CAS  Google Scholar 

  9. Yamasaki T., Kurokawa S., Watanabe K.I., Ikuta K., Ohama T. 2005. Shared molecular characteristics of successfully transformed mitochondrial genomes in Chlamydomonas reinhardtii. Plant Mol. Biol. 58, 515–27.

    Article  PubMed  CAS  Google Scholar 

  10. Remacle C., Cardol P., Coosemans N., Gaisne M., Bonnefoy N. 2006. High-efficiency biolistic transformation of Chlamydomonas mitochondria can be used to insert mutations in complex I genes. Proc. Natl. Acad. Sci. USA. 103, 4771–4776.

    Article  PubMed  CAS  Google Scholar 

  11. Klingenberg M. 1980. The ADP-ATP translocation in mitochondria, a membrane potential controlled transport. J. Membrane Biol. 56, 97–105.

    Article  CAS  Google Scholar 

  12. Gropp T., Brustovetsky N., Klingenberg M., Muller V., Fendler K., Bamberg E. 1999. Kinetics of electrogenic transport by the ADP/ATP carrier. Biophys. J. 77, 714–726.

    Article  PubMed  CAS  Google Scholar 

  13. Huber T., Klingenberg M., Beyer K. 1999. Binding of nucleotides by the mitochondrial ADP/ATP carrier as studied by 1H nuclear magnetic resonance spectroscopy. Biochemistry. 38, 762–769.

    Article  PubMed  CAS  Google Scholar 

  14. Konstantinov Y.M., Podsosonny V.A., Lutcenko G.N. 1988. Inhibition of adenine nucleotide translocation in maize seedling mitochondria by anionic detergents. Physiol. Plantarum. 72, 403–406.

    Article  CAS  Google Scholar 

  15. Zorov D.B. 1996. Mitochondrial transport of nucleic acids. Participation of benzodiazepine receptor. Biochemistry (Moscow) (Rus.). 61(7), 1320–1332.

    CAS  Google Scholar 

  16. Zorov D.B. 1996. Mitochondrial damage as a source of diseases and ageing: A strategy of how to fight these. Biochim. Biophys. Acta. 1275, 10–15.

    Article  PubMed  Google Scholar 

  17. Koulintchenko M., Temperley R.J., Mason P.A., Dietrich A., Lightowers R.N. 2006. Natural competence of mammalian mitochondria allows the molecular investigation of mitochondrial gene expression. Human Mol. Genet. 15, 143–154.

    Article  CAS  Google Scholar 

  18. Weber-Lotfi F., Ibrahim N., Boesch P., Cosset A., Konstantinov Yu., Lightowlers R.N., Dietrich A. 2009. Developing a genetic approach to investigate the mechanism of mitochondrial competence for DNA import. Biochim. Biophys. Acta. 1787, 320–327.

    Article  PubMed  CAS  Google Scholar 

  19. Patrushev M., Kasymov V., Patrusheva V., Ushakova T., Gogvadze V., Gaziev A. 2004. Mitochondrial permeability transition triggers the release of mtDNA fragments. Cell. Mol. Life Sci. 61, 3100–3103.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of the Russian Academy of Sciences, ul. Lermontova, 132, Irkutsk, 664033, Russia

    E. S. Klimenko & Yu. M. Konstantinov

  2. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, prosp. Lavrent’yeva, 8, Novosibirsk, 630090, Russia

    V. A. Mileiko, E. S. Morozkin & P. P. Laktionov

Authors
  1. E. S. Klimenko
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  2. V. A. Mileiko
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  3. E. S. Morozkin
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  4. P. P. Laktionov
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  5. Yu. M. Konstantinov
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Correspondence to E. S. Klimenko.

Additional information

Original Russian Text © E.S. Klimenko, V.A. Mileiko, E.S. Morozkin, P.P. Laktionov, Yu.M. Konstantinov, 2011, published in Biologicheskie Membrany, 2011, Vol. 28, No. 3, pp. 199–205.

The article was translated by the authors.

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Klimenko, E.S., Mileiko, V.A., Morozkin, E.S. et al. Study of DNA import and export in potato (Solanum tuberosum) mitochondria using quantitative PCR. Biochem. Moscow Suppl. Ser. A 5, 170–176 (2011). https://doi.org/10.1134/S1990747811030044

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  • DOI: https://doi.org/10.1134/S1990747811030044

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