Role of P-selectin and PSGL-1 in coagulation and thrombosis

 

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Summary

P-selectin and PSGL-1 are cell adhesion molecules, regulating the initial interactions between leukocytes and the blood vessel wall as well as between activated platelets and leukocytes. P-selectin is expressed on activated endothelial cells and platelets, while its major ligand PSGL-1 is expressed on leukocytes. Multiple studies have shown that these adhesion molecules are required for the normal recruitment of leukocytes during an inflammatory reaction. More recently, these adhesion molecules have been implicated in recruitment of leukocytes and leukocyte microparticles to thrombi. The P-selectinand PSGL-1-dependent delivery of circulating microparticles to thrombi appears to be important for normal tissue factor accumulation and fibrin generation in thrombi.

• References

• 1 Furie B, Furie BC. Molecular and cellular biology of blood coagulation. N Engl J Med 1992; 326: 800-6.
  CrossrefPubMedGoogle Scholar
• 2 Hsu-Lin S, Berman CL, Furie BC. et al. A platelet membrane protein expressed during platelet activation and secretion. Studies using a monoclonal antibody specific for thrombinactivated platelets. J Biol Chem 1984; 259: 9121-6.
  PubMedGoogle Scholar
• 3 McEver RP, Martin MN. A monoclonal antibody to a membrane glycoprotein binds only to activated platelets. J Biol Chem 1984; 259: 9799-804.
  PubMedGoogle Scholar
• 4 Bevilacqua MP, Stengelin S, Gimbrone Jr MA. et al. Endothelial leukocyte adhesion molecule 1: an inducible receptor for neutrophils related to complement regulatory proteins and lectins. Science 1989; 243: 1160-5.
  CrossrefPubMedGoogle Scholar
• 5 Johnston GI, Cook RG, McEver RP. Cloning of GMP-140, a granule membrane protein of platelets and endothelium: sequence similarity to proteins involved in cell adhesion and inflammation. Cell 1989; 56: 1033-44.
  CrossrefPubMedGoogle Scholar
• 6 Lasky LA, Singer MS, Yednock TA. et al. Cloning of a lymphocyte homing receptor reveals a lectin domain. Cell 1989; 56: 1045-55.
  CrossrefPubMedGoogle Scholar
• 7 Stenberg PE, McEver RP, Shuman MA. et al. A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation. J Cell Biol 1985; 101: 880-6.
  CrossrefPubMedGoogle Scholar
• 8 Berman CL, Yeo EL, Wencel-Drake JD. et al. A platelet alpha granule membrane protein that is associated with the plasma membrane after activation. Characterization and subcellular localization of platelet activationdependent granule-external membrane protein. J Clin Invest 1986; 78: 130-7.
  CrossrefPubMedGoogle Scholar
• 9 Bonfanti R, Furie BC, Furie B. et al. PADGEM (GMP140) is a component of Weibel-Palade bodies of human endothelial cells. Blood 1989; 73: 1109-12.
  PubMedGoogle Scholar
• 10 McEver RP, Beckstead JH, Moore KL. et al. GMP-140, a platelet alpha-granule membrane protein, is also synthesized by vascular endothelial cells and is localized in Weibel-Palade bodies. J Clin Invest 1989; 84: 92-9.
  CrossrefPubMedGoogle Scholar
• 11 Larsen E, Celi A, Gilbert GE. et al. PADGEM protein: a receptor that mediates the interaction of activated platelets with neutrophils and monocytes. Cell 1989; 59: 305-12.
  CrossrefPubMedGoogle Scholar
• 12 Mayadas TN, Johnson RC, Rayburn H. et al. Leukocyte rolling and extravasation are severely compromised in P selectin-deficient mice. Cell 1993; 74: 541-54.
  CrossrefPubMedGoogle Scholar
• 13 Ley K, Bullard DC, Arbones ML. et al. Sequential contribution of L- and P-selectin to leukocyte rolling in vivo. J Exp Med 1995; 181: 669-75.
  CrossrefPubMedGoogle Scholar
• 14 Subramaniam M, Saffaripour S, Watson SR. et al. Reduced recruitment of inflammatory cells in a contact hypersensitivity response in P-selectin-deficient mice. J Exp Med 1995; 181: 2277-82.
  CrossrefPubMedGoogle Scholar
• 15 Larsen GR, Sako D, Ahern TJ. et al. P-selectin and E-selectin. Distinct but overlapping leukocyte ligand specificities. J Biol Chem 1992; 267: 11104-10.
  PubMedGoogle Scholar
• 16 Sako D, Chang XJ, Barone KM. et al. Expression cloning of a functional glycoprotein ligand for P-selectin. Cell 1993; 75: 1179-86.
  CrossrefPubMedGoogle Scholar
• 17 Yang J, Galipeau J, Kozak CA. et al. Mouse Pselectin glycoprotein ligand-1: molecular cloning, chromosomal localization, and expression of a functional P-selectin receptor. Blood 1996; 87: 4176-86.
  PubMedGoogle Scholar
• 18 Sako D, Comess KM, Barone KM. et al. A sulfated peptide segment at the amino terminus of PSGL-1 is critical for P-selectin binding. Cell 1995; 83: 323-31.
  CrossrefPubMedGoogle Scholar
• 19 Wilkins PP, Moore KL, McEver RP. et al. Tyrosine sulfation of P-selectin glycoprotein ligand-1 is required for high affinity binding to P-selectin. J Biol Chem 1995; 270: 22677-80.
  CrossrefPubMedGoogle Scholar
• 20 Li F, Wilkins PP, Crawley S. et al. Posttranslational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to Pand E-selectin. J Biol Chem 1996; 271: 3255-64.
  CrossrefPubMedGoogle Scholar
• 21 Moore KL, Patel KD, Bruehl RE. et al. P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin. J Cell Biol 1995; 128: 661-71.
  CrossrefPubMedGoogle Scholar
• 22 Laszik Z, Jansen PJ, Cummings RD. et al. P-selectin glycoprotein ligand-1 is broadly expressed in cells of myeloid, lymphoid, and dendritic lineage and in some nonhematopoietic cells. Blood 1996; 88: 3010-21.
  PubMedGoogle Scholar
• 23 Yang J, Hirata T, Croce K. et al. Targeted gene disruption demonstrates that P-selectin glycoprotein ligand 1 (PSGL-1) is required for P-selectin-mediated but not E-selectinmediated neutrophil rolling and migration. J Exp Med 1999; 190: 1769-82.
  CrossrefPubMedGoogle Scholar
• 24 Hirata T, Merrill-Skoloff G, Aab M. et al. P-Selectin glycoprotein ligand 1 (PSGL-1) is a physiological ligand for E-selectin in mediating T helper 1 lymphocyte migration. J Exp Med 2000; 192: 1669-76.
  CrossrefPubMedGoogle Scholar
• 25 Hirata T, Furie BC, Furie B. P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin. J Immunol 2002; 169: 4307-13.
  CrossrefPubMedGoogle Scholar
• 26 Palabrica TM, Furie BC, Konstam MA. et al. Thrombus imaging in a primate model with antibodies specific for an external membrane protein of activated platelets. Proc Natl Acad Sci U S A 1989; 86: 1036-40.
  CrossrefPubMedGoogle Scholar
• 27 Palabrica T, Lobb R, Furie BC. et al. Leukocyte accumulation promoting fibrin deposition is mediated in vivo by P-selectin on adherent platelets. Nature 1992; 359: 848-51.
  CrossrefPubMedGoogle Scholar
• 28 Deykin D. Thrombogenesis. N Engl J Med 1967; 276: 622-8.
  CrossrefPubMedGoogle Scholar
• 29 Semeraro N, Biondi A, Lorenzet R. et al. Direct induction of tissue factor synthesis by endotoxin in human macrophages from diverse anatomical sites. Immunology 1983; 50: 529-35.
  PubMedGoogle Scholar
• 30 Colucci M, Balconi G, Lorenzet R. et al. Cultured human endothelial cells generate tissue factor in response to endotoxin. J Clin Invest 1983; 71: 1893-6.
  CrossrefPubMedGoogle Scholar
• 31 Niemetz J, Marcus AJ. The stimulatory effect of platelets and platelet membranes on the procoagulant activity of leukocytes. J Clin Invest 1974; 54: 1437-43.
  CrossrefPubMedGoogle Scholar
• 32 Pinder PB, Hunt JA, Zacharski LR. In vitro stimulation of monocyte tissue factor activity by autologous platelets. Am J Hematol 1985; 19: 317-25.
  CrossrefPubMedGoogle Scholar
• 33 Celi A, Pellegrini G, Lorenzet R. et al. Pselectin induces the expression of tissue factor on monocytes. Proc Natl Acad Sci USA 1994; 91: 8767-71.
  CrossrefPubMedGoogle Scholar
• 34 Zumbach M, Hofmann M, Borcea V. et al. Tissue factor antigen is elevated in patients with microvascular complications of diabetes mellitus. Exp Clin Endocrinol Diabetes 1997; 105: 206-12.
  Article in Thieme ConnectPubMedGoogle Scholar
• 35 Koyama T, Nishida K, Ohdama S. et al. Determination of plasma tissue factor antigen and its clinical significance. Br J Haematol 1994; 87: 343-7.
  CrossrefPubMedGoogle Scholar
• 36 Fareed J, Callas DD, Hoppensteadt D. et al. Tissue factor antigen levels in various biological fluids. Blood Coagul Fibrinolysis 1995; (Suppl. 06) (01) S32-6.
  PubMedGoogle Scholar
• 37 Suefuji H, Ogawa H, Yasue H. et al. Increased plasma tissue factor levels in acute myocardial infarction. Am Heart J 1997; 134: 253-9.
  CrossrefPubMedGoogle Scholar
• 38 Giesen PL, Rauch U, Bohrmann B. et al. Blood-borne tissue factor: another view of thrombosis. Proc Natl Acad Sci U S A 1999; 96: 2311-5.
  CrossrefPubMedGoogle Scholar
• 39 Rauch U, Bonderman D, Bohrmann B. et al. Transfer of tissue factor from leukocytes to platelets is mediated by CD15 and tissue factor. Blood 2000; 96: 170-5.
  PubMedGoogle Scholar
• 40 Andre P, Hartwell D, Hrachovinova I. et al. Pro-coagulant state resulting from high levels of soluble P-selectin in blood. Proc Natl Acad Sci U S A 2000; 97: 13835-40.
  CrossrefPubMedGoogle Scholar
• 41 Sullivan VV, Hawley AE, Farris DM. et al. Decrease in fibrin content of venous thrombi in selectin-deficient mice. J Surg Res 2003; 109: 1-7.
  CrossrefPubMedGoogle Scholar
• 42 Falati S, Gross P, Merrill-Skoloff G. et al. Real-time in vivo imaging of platelets, tissue factor and fibrin during arterial thrombus formation in the mouse. Nat Med 2002; 08: 1175-81.
  CrossrefPubMedGoogle Scholar
• 43 Kurz KD, Main BW, Sandusky GE. Rat model of arterial thrombosis induced by ferric chloride. Thromb Res 1990; 60: 269-80.
  CrossrefPubMedGoogle Scholar
• 44 Rosen ED, Raymond S, Zollman A. et al. Laser-induced noninvasive vascular injury models in mice generate platelet- and coagulation-dependent thrombi. Am J Pathol 2001; 158: 1613-22.
  CrossrefPubMedGoogle Scholar
• 45 Myers Jr D, Farris D, Hawley A. et al. Selectins influence thrombosis in a mouse model of experimental deep venous thrombosis. J Surg Res 2002; 108: 212-21.
  CrossrefPubMedGoogle Scholar
• 46 Myers DD, Hawley AE, Farris DM. et al. P-selectin and leukocyte microparticles are associated with venous thrombogenesis. J Vasc Surg 2003; 38: 1075-89.
  CrossrefPubMedGoogle Scholar
• 47 Hartwell DW, Mayadas TN, Berger G. et al. Role of P-selectin cytoplasmic domain in granular targeting in vivo and in early inflammatory responses. J Cell Biol 1998; 143: 1129-41.
  CrossrefPubMedGoogle Scholar
• 48 Falati S, Liu Q, Gross P. et al. Accumulation of tissue factor into developing thrombi in vivo is dependent upon microparticle P-selectin glycoprotein ligand 1 and platelet P-selectin. J Exp Med 2003; 197: 1585-98.
  CrossrefPubMedGoogle Scholar
• 49 Gross P, Furie BC, Furie B. Kinetics of platelet activation, P-selectin expression and leukocyte rolling during arterial thrombus formation in vivo. Blood. 2002 100.
  PubMedGoogle Scholar
• 50 Chou J, Mackman N, Merrill-Skoloff G. et al. Hematopoietic cell-derived microparticle tissue factor constributes to fibrin formation during thrombus propagation. Blood 2004; 92: 621-6.
  PubMedGoogle Scholar
• 51 Dunlop LC, Skinner MP, Bendall LJ. et al. Characterization of GMP-140 (P-selectin) as a circulating plasma protein. J Exp Med 1992; 175: 1147-50.
  CrossrefPubMedGoogle Scholar
• 52 Fijnheer R, Frijns CJ, Korteweg J. et al. The origin of P-selectin as a circulating plasma protein. Thromb Haemost 1997; 77: 1081-5.
  Article in Thieme ConnectPubMedGoogle Scholar
• 53 Berger G, Hartwell DW, Wagner DD. P-Selectin and platelet clearance. Blood 1998; 92: 4446-52.
  CrossrefPubMedGoogle Scholar
• 54 Michelson AD, Barnard MR, Hechtman HB. et al. In vivo tracking of platelets: circulating degranulated platelets rapidly lose surface P-selectin but continue to circulate and function. Proc Natl Acad Sci U S A 1996; 93: 11877-82.
  CrossrefPubMedGoogle Scholar
• 55 Hrachovinova I, Cambien B, Hafezi-Moghadam A. et al. Interaction of P-selectin and PSGL-1 generates microparticles that correct hemostasis in a mouse model of hemophilia A. Nat Med 2003; 09: 1020-5.
  CrossrefPubMedGoogle Scholar