Screening Analysis of the Pathogenic Gene of Dentin Dysplasia Type I

Objective : Pathogenic genes of Dentin Dysplasia Type I (DD-I) were selected through full exon scanning. Methods : The full peripheral blood DNA of two patients with DD-I was extracted for exon group sequencing. According to exon sequencing results and overlap method, bioinformatics analysis was performed. The suspicious pathogenic loci mentioned in previous studies were excluded, and candidate loci of exon group sequencing were selected and sequenced based on gene function. Pathogenic loci with coseparation were screened in pedigree in turn and the mutant gene was searched. Results : 101 candidate genes were screened after comparison of common candidate mutation sites between the two samples. Conclusion : The candidate pathogenic genes of DD-I were screened through full exon scanning, which provided certain experimental and theoretical basis for the study and treatment of DD-I.