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Effect of Human Endothelial Progenitor Cell (EPC)- or Mouse Vascular Endothelial Growth Factor-Derived Vessel Formation on the Survival of Vitrified/Warmed Mouse Ovarian Grafts

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Abstract

The aim of this study was to evaluate the effectiveness of improving angiogenesis at graft sites on the survival of follicles in transplanted ovarian tissue. Matrigel containing 5 × 105 of cord blood-derived endothelial progenitor cells (EPCs) or 200 ng of mouse vascular endothelial growth factor (VEGF) was injected subcutaneously into BALB/c-Nu mice. After 1 week, vitrified/warmed ovaries from female B6D2F1 mice were subcutaneously transplanted into the injection sites. After 1, 2, and 4 weeks posttransplantation, the ovaries were recovered and subjected to histological analysis. Oocytes were collected from the transplanted ovaries, and their fertilization, embryonic development, and delivery were also observed. Vitrified/warmed ovaries transplanted into EPC- or VEGF-treated sites developed more blood vessels and showed better follicle survival than those transplanted into sham-injected sites. Normal embryonic development and consequent live births were obtained using oocytes recovered from cryopreserved/transplanted ovaries. Treatment with EPCs or VEGF could prevent the ischemic damage during the early revascularization stage of ovarian transplantation.

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Authors and Affiliations

  1. Fertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, 606-5 Yeoksam-dong, Gangnam-gu, Seoul, 135-081, South Korea

    Soo Kyung Cha MS, Dong Hyuk Shin BS, Bo Yeun Kim MS, Sook-Young Yoon PhD, Tae Ki Yoon MD, Woo Sik Lee MD, PhD & Dong Ryul Lee PhD

  2. Department of Biomedical Science, College of Life Science, CHA University, Seoul, Korea

    Soo Kyung Cha MS, Sook-Young Yoon PhD & Dong Ryul Lee PhD

  3. Department of Stem Cell Biology, School of Medicine, Konkuk University, Seoul, 143-701, Korea

    Hyung Min Chung PhD

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  1. Soo Kyung Cha MS
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  2. Dong Hyuk Shin BS
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Correspondence to Hyung Min Chung PhD or Dong Ryul Lee PhD.

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Cha, S.K., Shin, D.H., Kim, B.Y. et al. Effect of Human Endothelial Progenitor Cell (EPC)- or Mouse Vascular Endothelial Growth Factor-Derived Vessel Formation on the Survival of Vitrified/Warmed Mouse Ovarian Grafts. Reprod. Sci. 21, 859–868 (2014). https://doi.org/10.1177/1933719113518983

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  • DOI: https://doi.org/10.1177/1933719113518983

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