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Expression and Subcellular Localization of Metastasis-Associated Protein 1, Its Short Form, and Estrogen Receptors in Rat Placenta

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Abstract

Metastasis-associated protein 1 (MTA1) and its short form (MTA1s) regulate the function of estrogen receptors (ERs). Estrogens, via ERs, affect placental growth and fetal development, a process that may involve MTA1 signaling. Expression of MTA1, MTA1s, ERα, and ERβ genes and proteins in rat placentas was studied on 16, 19, and 21 days of gestation (dg). The ERβ messenger RNA decreased significantly toward the end of gestation, whereas its protein level increased in the nuclear fraction on 21 dg. Both MTA1 and MTA1s increased with gestation. Decidual, trophoblast giant, glycogen, and villous trophoblast cells expressed MTA1, ERα, and ERβ proteins on all dg with colocalization of MTA1 with ERα and ERβ in the nucleus and cytoplasm. Expression of MTA1 suggests a possible role in regulating placental functions; considering the repressive function of MTA1 on ERs, the expression of MTA1 suggests that placental cells may be less sensitive to estrogens during late pregnancy.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, Kuwait University, P.O. Box 24923, 13110, Safat, Kuwait

    Maie Dawoud Al-Bader PhD & Aseel El-Farra MSc

  2. Department of Anatomy, Faculty of Medicine, Kuwait University, Kuwait

    Narayana Kilarkaje PhD

  3. Department of Pathology, Faculty of Medicine, Kuwait University, Kuwait

    Abeer Adnan Al-Abdallah PhD

Authors
  1. Maie Dawoud Al-Bader PhD
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  2. Narayana Kilarkaje PhD
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  3. Aseel El-Farra MSc
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  4. Abeer Adnan Al-Abdallah PhD
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Correspondence to Maie Dawoud Al-Bader PhD.

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Al-Bader, M.D., Kilarkaje, N., El-Farra, A. et al. Expression and Subcellular Localization of Metastasis-Associated Protein 1, Its Short Form, and Estrogen Receptors in Rat Placenta. Reprod. Sci. 22, 484–494 (2015). https://doi.org/10.1177/1933719114549851

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  • DOI: https://doi.org/10.1177/1933719114549851

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