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Progesterone-Induced miR-152 Inhibits the Proliferation of Endometrial Epithelial Cells by Downregulating WNT-1

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Abstract

Progesterone (P4) is an important ovarian hormone that inhibits estrogen-dependent proliferation of endometrial epithelial cells (EECs). miR-152 has been reported to be a cell cycle regulator. In this study, we first demonstrated that P4 induced the expression of miR-152 in ovariectomized mice and Ishikawa cell. miR-152 was detected in the human endometrial cell lines that were stably transfected with P4 receptor. Results showed that P4 induced its expression through its receptor B subtype. Then, using the specific miRNA mimic and inhibitor, we proved that miR-152 impeded G1/S transition in the cell cycle of EECs and inhibited cellular proliferation via downregulating WNT-1 in mice and human endometrial cancer cell lines (Ishikawa, HEC-1-b, and KLE). miR-152 induced by P4 is an important inhibitor for the proliferation of EECs. miR-152 may be an important tumor suppressor microRNA in endometrial cancer.

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Authors and Affiliations

  1. Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, No. 17, Section 3, Renmin South Road, Chengdu, 610041, People’s Republic of China

    Li Nie MD, You-bo Zhao MD, Jun-li Pan MD, Yi Lei MD, Min Liu PhD, Yun Long PhD, Jin-hu Zhang BS, Ming-qian Xu BS, Dong-zhi Yuan PhD & Li-min Yue PhD

  2. Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, People’s Republic of China

    Ying Hu PhD

Authors
  1. Li Nie MD
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  2. You-bo Zhao MD
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  3. Jun-li Pan MD
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  4. Yi Lei MD
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  5. Min Liu PhD
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  6. Yun Long PhD
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  7. Jin-hu Zhang BS
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  8. Ying Hu PhD
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  9. Ming-qian Xu BS
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  10. Dong-zhi Yuan PhD
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  11. Li-min Yue PhD
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Corresponding authors

Correspondence to Dong-zhi Yuan PhD or Li-min Yue PhD.

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Nie, L., Zhao, Yb., Pan, Jl. et al. Progesterone-Induced miR-152 Inhibits the Proliferation of Endometrial Epithelial Cells by Downregulating WNT-1. Reprod. Sci. 24, 1444–1453 (2017). https://doi.org/10.1177/1933719116689595

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