The growing number of the elderly population and their complex health problems reveal the necessity of creating adequate health care programs. Among the elderly, the prevalence of hypertension reaches about 50% and has been considered the main risk factor for cardiovascular diseases. Therefore, the comprehension of the different mechanisms that generate hypertension is fundamental for creating strategies to control high blood pressure (BP).

It has been shown that elevated uric acid (UA) can be one of the mechanisms responsible for hypertension. It is the final enzymatic and product of purine degradation in the human body and it is catalyzed by xanthine oxidase [1]. Some authors have shown that low mitochondrial capacity can increase xanthine oxidase activity which will further induce UA formation, thus favouring anion superoxide (O₂ ^-.) production [2] which can lead to endothelial dysfunction and vascular damage [3, 4]. Elevated levels of UA can be associated with NO-scavenging, lower vasodilator response [5] and higher BP levels [6]. Accordingly, Zharikov et al. [7] showed that almost 80% of patients with pulmonary hypertension had hyperuricemia. However, regular physical exercise has been considered the major stimulus to prevent and/or control high BP [8, 9]. Briefly, physical exercise promotes an improvement in metabolic syndrome [10], autonomic modulation (heart-rate variability) [11], nitric oxide concentration and oxidative stress [4, 9], lipid profile [12] and other disorders. Nevertheless, the effectiveness of regular exercise on UA content and its relationship with hypertension is still controversial, mainly because of the differences among type, intensity, frequency and duration of the exercise programs found in the literature [13‐15], especially those related to elderly [5, 14, 16].

In addition, the current recommendation for elderly people has been recognized as a combination of different types of exercise for maintaining physical functional fitness [14]. Both the American College of Sports Medicine [14] and the American Heart Association (AHA) [15, 16] recommend that the exercise prescription for adults and elderly people should include endurance, muscle strengthening as well as balance and flexibility exercise (also called multicomponent exercises).

Following these recommendations, some studies compared the effects of a multicomponent exercise program with a strength exercise program [12] and with a walking program [13]. These studies suggested that better results on lipid profile can be found after a multicomponent exercise program compared to others. Therefore, it seems reasonable to suggest that results from a multicomponent functional fitness test are more appropriate to define training status instead of the evaluation of only one component of physical fitness, like cardiovascular capacity [12, 17].

Although it has been suggested that people with sedentary lifestyle exhibit lower mitochondrial capacity [18], there is limited evidence about the relationship among training status, BP and UA. Thus, the hypothesis of this study was that people with better training status could have lower BP and UA levels. Therefore, the purpose of this study was to investigate the relationship between BP and UA and whether this relationship may be mediated by the training status.

From all the clusters visited, 123 participants met our initial inclusion criteria (17 men and 106 women). All participants were separated according to the GFFI index (G1 = 207.3 ± 62.7 / G2 = 351.2 ± 28.6 / G3 = 437.2 ± 23.2). The groups were composed of both genders even though the number of men was very low. The option to use the GFFI as an independent variable was because it comprises a multicomponent assessment, had a good correlation with VO₂max (Figure 1), and also correlated with more variables than VO₂max, as shown in Table 1. Therefore, this index seems to be more adequate to indicate the training status.

Pearson’s correlation coefficient also showed moderate correlation between UA and SBP (r=0.3/p<0.001) and DBP (r=0.29/p<0.001). Moreover, participants with elevated UA also exhibited high blood pressure values (Figure 2). Although BMI and age were considered as confounding factors, when participants were divided according to the UA levels the values of BMI (26.7 ± 4.0 and 28.5 ± 3.8) and age (56.7 ± 9.8 and 59.4 ± 11.6) were similar in both groups (low and high level of UA respectively).

In order to demonstrate how training status can mediate the relationship between blood pressure and uric acid, all participants were divided into three groups according the GFFI results. However, due to the difference among groups in BMI (G1 = 28.2 ± 4.2 / G2 = 27.4 ± 3.5 / G3 = 24.6 ± 3.5) and age (G1 = 60.9 ± 11.7 / G2 = 55.3 ± 8.1 / G3 = 53.9 ± 9.1) General Linear Model was performed to adjust for BMI and age. Subjects’ characteristics are presented in Table 2.

As expected, the VO₂max was different among groups. As shown in table 2, the values of VO₂max in G3 were higher than those found in G1.

For lipid profile, CHOL and TG values were significantly lower between G2 and G3 compared to G1 and no difference was found in LDL-c and HDL-c among groups Figures 3 and 4 show BP and UA results. As shown in Figure 3, G2 and G3 presented lower values of SBP and DBP compared to G1. However, UA results were not different among groups (Figure 4).

Nitrite concentration and oxidative stress are other important variables that can explain the relationship between BP and UA Figure 5 illustrates that nitrite concentration was higher in G3 compared to G2, and G2 which was higher than G1. No difference was found in T-BARS analysis among groups.

Although the results indicate a relationship between the variables, the absence of differences in UA levels (Figure 4) could raise doubts regarding this relationship. Thus, Table 3 shows the results of BP when participants were divided according to the GFFI and UA.

It can be observed that only in G3 different levels of UA resulted in different values in BP. Therefore, this result suggests that very good levels of training status determine a reduction in BP values in the participants with low levels of UA.

The present study was designed to investigate the potential effect of a good level of training status on blood pressure and UA concentration and the main results found were that patients with higher training status and low level of uric acid have low values of blood pressure.

Uric Acid has an important influence on vascular control by increasing oxidative stress (O₂ ^-. production) and NO-scavenging. This mechanism can affect the vasodilatation thereby increasing BP. Although it has been reported that physical exercise contributes to an adequate maintenance of BP, there is limited evidence demonstrating the effect of physical exercise on UA concentration.

Our results showed that subjects with elevated UA concentration demonstrated elevated BP. Also, we demonstrated that this result can be mediated by training status, since subjects with higher training status and lower levels of UA presented lower values of BP. The mechanisms that may explain the association between UA concentration and BP are still unknown. The anion superoxide (O₂ ^·­) produced during the UA formation acts as a NO-scavenging thus reducing the NO bioavailability. NO is a potent vasodilator and has an important function in BP control [27‐30]. This is in agreement with Zharikov et al. [7], who have shown a widespread effect of inhibiting vascular endothelial NO production and accumulation of cyclic guanosine monophosphate (cGMP) without effect on endothelial nitric oxide synthase (eNOS) activity and expression. In accordance, Corry et al. (2008) have also demonstrated in cell cultures that the vascular smooth muscle cells from rat aorta decrease metabolic concentration of nitrite and nitrate and increase hydrogen peroxide (H₂O₂) and 8-isoprostane activity when UA (200 μmol) was added. The increased production of these compounds suggests that UA stimulates the production of reactive oxygen species (ROS) which are associated with impaired endothelium-dependent vasodilatation. Therefore, UA seems to be associated with decreased NO bioavailability and consequently increased BP [6]. In accordance, we have shown that nitric content was higher in the patients with high training status.

The results of this study showed no difference in T-BARS and UA concentration among groups. However, high level of training status resulted in better results of nitrite concentration and systolic and diastolic BP. Therefore, the mechanism responsible for better control of BP may be associated with better antioxidant capacity achieved by higher levels of training status and consequently, higher nitric oxide bioavailability.

It is worth noting that higher level of training status can only be achieved with regular physical training which is able to promote improvement in the antioxidant system. In fact, multicomponent exercise, including aerobic exercise, can enhance the mitochondrial respiration and it might contribute to a lower ADP/ATP ratio in skeletal muscle [31]. When ATP is used during exercise, AMP is degraded to IMP, hypoxanthine, xanthine and finally to UA. During exercise there is an increase of xanthine oxidase that contributes to the oxidative stress. However, this effect can be reduced by training status [32].

Waring et al. (2003) divided the participants with normal BP into two groups. The first group performed moderate-intensity exercise and the values of BP 40 min post-exercise were 147(6)/101(5) mmHg (SBP/DBP). The second group received oral administration of UA (0.5 g) before acute exercise and the values of BP 40 min post-exercise were 153(6)/103(6) mmHg (SBP/DBP). This study showed that an increased UA concentration might contribute to an increased BP after exercise. However, moderate-intensity exercise up-regulated the xanthine oxidase activity suggesting that a physiological level of UA might contribute to the BP control, especially because of the improvements in the antioxidative capacity [33].

Although regular exercise has been considered the major stimulus to prevent high blood pressure, there is still no consensus of which is the best combination among type, intensity and frequency to achieve such benefits. Thus, our study leads us to the discussion of how training status, regardless of how physical exercise was previously performed, exerts an important role on this mechanism associated with BP control.

The results presented in Table 2 showed that a higher level of functional fitness (G3) had a marked effect on CHOL and TG, when compared to a lower level of functional fitness (G1). Although LDL-c and HDL-c did not present significant difference among groups, lower results in LDL-c and higher results in HDL-c can be observed when training status is increased, suggesting that high GFFI may contribute to decrease the risk factor for cardiovascular disease. The lack of differences in LDL-c and HDL-c among groups can also be explained by the reference range established by the AHA [16]. It is important to note that the participants evaluated in this study did not present cardiovascular disease, and the values of lipid profile were within a range considered normal or borderline. Therefore, marked differences were not expected for these variables. However, it is important to assume that these effects would be very important for people with cardiovascular disease. Marques et al. [12] showed better results with multicomponent evaluation in TG and HDL-c when compared to one component (endurance). Kemmler et al. [10] also showed that a multipurpose exercise program significantly affected most parameters such as CHO, TG and HDL-c of the metabolic syndrome in elderly women. In addition, numerous physiological mechanisms may explain this alteration such as increased skeletal muscle lipoprotein lipase activity and increased capillary density [12, 34].

The present study found a relationship among SBP, DBP, UA, lipid profile and GFFI. Taking together the findings that subjects with elevated values of UA and lower values of nitrite had higher values of BP, this study suggests that the relationship between blood pressure and the oxidative stress produced by acid uric may be mediated by training status.

The authors understand that ambulatory BP measurements are more accurate than office BP measurements. However, we did not have this technique available at the time the data were collected; (b) although the authors are aware of the differences between genders, men and women were not analyzed separated in our study because no differences in all variables were found between male and female. We recognize the low number of men participating in the study; (c) the antihypertensive drugs were not interrupted during the study, however, participants were asked not to take the medicine before the blood pressure measurements; (d) some blood analysis such insulin levels were not performed; (e) no specific control of diet was performed, however, participants were requested to have a light meal before the test.