Background
Methods
Participants
Immunohistochemistry
Reverse transcription–polymerase chain reaction quantification of CD24 and CD44
Determination of H. pyloriinfection
Statistical analysis
Results
CD24 and CD44 expression levels in gastric cancer
Correlations between CD24/CD44 expression levels and clinicopathologic parameters
CD24 positive rate n (%) |
P value
| CD44 positive rate n (%) |
P value
| |
---|---|---|---|---|
Gender | ||||
Male (n=221) | 92(41.6) | 0.148 | 133(60.2) | 0.835 |
Female (n=69) | 22(31.9) | 45(65.2) | ||
Age(y) | ||||
≤60 (n=127) | 48(37.8) | 0.435 | 85(66.9) | 0.087 |
>60 (n=163) | 69(42.3) | 93(57.0) | ||
H.pylori infection (n=103) | ||||
Positive (n=67) | 24(35.8) | 0.096 | 48(71.6) | 0.815 |
Negative (n=36) | 19(52.8) | 25(69.4) | ||
Lauren classification | ||||
Intestinal type (n=104) | 42(40.3) | 0.965 | 67(64.4) | 0.168 |
Diffuse type (n=186) | 77(41.3) | 103(55.3) | ||
WHO classification | ||||
Papillary adenocarcinoma (n=4) | 1(25.0) | 0.906 | 4(100.0) | 0.064 |
Tubular adenocarcinoma | ||||
Well (n=1) | 0(0.0) | 0(0.0) | ||
Moderate (n=98) | 30(30.6) | 49(50.0) | ||
Poor (n=153) | 47(30.7) | 87(56.9) | ||
Mucinous adenocarcinoma (n=27) | 7(25.9) | 13(48.1) | ||
Poorly cohesive carcinomas (Signet ring cell carcinomas) (n=7) | 1(14.3) | 1(14.3) | ||
Lymph-vascular invasion | ||||
Absent (n=134) | 56(41.8) | 0.642 | 72(53.7) | 0.013 |
Present (n=156) | 61(39.1) | 106(67.9) | ||
TNM stage | ||||
I (n=23) | 10(43.5) | 0.844 | 16(69.6) | 0.559 |
II (n=47) | 19(40.4) | 32(68.1) | ||
III (n=187) | 77(41.2) | 110(58.9) | ||
IV (n=33) | 11(33.3) | 20(60.6) | ||
Invasion | ||||
T1 (n=9) | 2(22.2) | 0.537 | 7(77.8) | 0.485 |
T2 (n=36) | 14(38.9) | 22(61.1) | ||
T3 (n=212) | 85(40.1) | 132(62.2) | ||
T4 (n=33) | 16(48.5) | 17(51.5) | ||
Lymph node metastasis | ||||
N0 (n=62) | 27(43.5) | 0.445 | 40(64.5) | 0.534 |
N1 (n=89) | 39(43.8) | 49(55.1) | ||
N2 (n=74) | 24(32.4) | 47(63.5) | ||
N3 (n=65) | 27(41.5) | 42(64.6) | ||
Survival | ||||
Survived (n=171) | 62(36.3) | 0.089 | 96(56.1) | 0.028 |
Died (n=119) | 55(46.2) | 82(68.9) |
CD44 expression was associated with poor survival
CD44 expression was an independent prognostic marker
Relative risk (95% CI) |
P value*
| |
---|---|---|
CD44 expression | ||
Negative (n=112) | Reference | |
Positive (n=178) | 1.57(1.05-2.36) | 0.029 |
CD24 expression | ||
Negative (n=173) | Reference | |
Positive (n=117) | 1.41(0.98-2.02) | 0.065 |
TNM staging | ||
I (n=23) | Reference | |
II (n=47) | 1.69(0.46-6.24) | 0.433 |
III (n=187) | 4.32(1.33-14.01) | 0.015 |
IV (n=33) | 14.53(4.27-49.41) | <0.001 |
Lymph-vascular invasion | ||
Absent (n=134) | Reference | |
Present (n=156) | 1.60(1.09-2.35) | 0.016 |