Background
Methods
Cohort and collection of samples
Patients | Sex/age | Symptoms |
Blastocystis sp. ST by non-qPCRa
| Nucleotide differencesb
|
Blastocystissp. ST by qPCR | Accession no. |
---|---|---|---|---|---|---|
DSS1 | M/6.2 | Abdominal pain | 1 | KF848509 | ||
DSS2 | F/5.9 | Abdominal pain, diarrhea | 3 (Sc) | 0 | KF848510 | |
DSS3 | M/6.8 | 3 (Sc) | KF848511 | |||
DSS4 | F/6.1 | Abdominal pain, vomiting | 1 (Sc) | KF848512 | ||
DSS5 | M/8.3 | 3 | KF848513 | |||
DSS6 | M/7.3 | Abdominal pain, diarrhea | 1 (Sc) | KF848514 | ||
DSS7 | F/5.9 | 3 | KF848515 | |||
DSS8 | M/6.8 | Abdominal pain, vomiting | 1 (Sc) | KF848516 | ||
DSS9 | F/9.2 | Abdominal pain | 3 | KF848517 | ||
DSS10 | M/9.7 | Vomiting | 3 (Sc) | 0 | KF848518 | |
DSS11 | F/10 | Diarrhea, vomiting | 3 (Sc) | KF848519 | ||
DSS12 | M/8.8 | Abdominal pain, vomiting | 3 | KF848520 | ||
DSS13 | F/8.6 | Abdominal pain | 1 (Sc) | KF848521 | ||
DSS14 | M/7 | 2 (Sc) | 0 | KF848522 | ||
DSS15 | F/7.7 | 2 (Sc) | KF848523 | |||
DSS16 | M/6.9 | 3 | KF848524 | |||
DSS17 | F/7.7 | 3 | KF848525 | |||
DSS18 | F/6.1 | 3 (Sc) | 0 to14 | KF848526-7 | ||
DSS19 | M/8.7 | 2 | KF848528 | |||
DSS20 | M/9.7 | Abdominal pain | 2 (Sc) | KF848529 | ||
DSS21 | F/6 | Abdominal pain | 1 (Sc) | KF848530 | ||
DSS22 | M/10.1 | Abdominal pain | 3 (Sc) | KF848531 | ||
DSS23 | M/7.7 | Mixed 2 and 3 (P) | 0 | KF848532-3 | ||
DSS24 | F/6.4 | 1 (Sc) | KF848534 | |||
DSS25 | F/9.6 | Abdominal pain | Mixed 3 and 4 (Sc) | 0 to 4 | KF848535-7 | |
DSS26 | F/6.5 | Abdominal pain | Mixed 1, 2 and 3(Sc) | KF848538-40 | ||
DSS27 | M/7.2 | 3 (Sc) | KF848541 | |||
DSS28 | M/8 | 3 (Sc) | KF848542 | |||
DSS29 | F/7.3 | 2 | KF848543 | |||
DSS30 | M/5.8 | Abdominal pain | 1 | KF848544 | ||
DSS31 | F/8.6 | Abdominal pain | 2 (Sc) | KF848545 | ||
DSS32 | M/6.6 | 3 (Sc) | KF848546 | |||
DSS33 | M/7.7 | Abdominal pain | Mixed 2 and 3 (Sc) | 0 | KF848547-8 | |
DSS34 | M/9.5 | Vomiting | 3 (Sc) | KF848549 | ||
DSS35 | M/7.7 | Abdominal pain | 3 (Sc) | 0 | KF848550 | |
DSS36 | F/5.9 | Abdominal pain | 3 (Sc) | KF848551 | ||
DSS37 | M/7.4 | 2 (Sc) | 0 | KF848552 | ||
DSS38 | F/9.7 | Abdominal pain, diarrhea, vomiting | 1 (Sc) | 0 | KF848553 | |
DSS39 | F/9.8 | Abdominal pain | 3 | KF848554 | ||
DSS40 | F/9.9 | Abdominal pain, diarrhea, vomiting | 2 (Sc) | KF848555 | ||
DSS41 | F/9.7 | Vomiting | 3 (Sc) | 0 | KF848556 | |
DSS42 | F/10.2 | Abdominal pain, diarrhea | 1 (Sc) | KF848557 | ||
DSS43 | M/9.3 | 2 (Sc) | KF848558 | |||
DSS44 | M/10 | Abdominal pain | 3 (Sc) | KF848559 | ||
DSS45 | F/8.9 | Abdominal pain | 3 (Sc) | 0 to 14 | KF848560-1 | |
DSS46 | F/10.2 | Abdominal pain | 3 | KF848562 | ||
DSS47 | F/10 | 3 (Sc) | 0 | KF848563 | ||
DSS48 | M/9.8 | 3 (Sc) | 0 | KF848564 | ||
DSS49 | F/10 | 2 (Sc) | KF848565 | |||
DSS50 | F/10.2 | 1 | KF848566 | |||
DSS51 | M/9.4 | 1 (Sc) | KF848567 | |||
DSS52 | M/9 | Abdominal pain | 1 (Sc) | KF848568 | ||
DSS53 | F/8.1 | 1 (Sc) | KF848569 | |||
DSS54 | M/8.5 | Mixed 1 and 2 (Sc) | 0 | KF848570-1 | ||
DSS55 | M/9.1 | Abdominal pain | 3 (Sc) | KF848572 | ||
DSS56 | F/8.6 | 1 (Sc) | KF848573 | |||
DSS57 | F/9.7 | Abdominal pain | 2 (Sc) | KF848574 | ||
DSS58 | M/9.3 | Abdominal pain | 2 (Sc) | KF848575 | ||
DSS59 | M/8.9 | Abdominal pain | 3 (Sc) | KF848576 | ||
DSS60 | F/8.8 | 3 (Sc) | KF848577 | |||
DSS61 | M/9.9 | 1 | KF848578 | |||
DSS62 | F/9.5 | Abdominal pain | 1 | KF848579 | ||
DSS63 | M/8.5 | Abdominal pain | 1 (Sc) | KF848580 | ||
DSS64 | M/6.2 | Abdominal pain | 3 (Sc) | KF848581 | ||
DSS65 | M/9.8 | 1 (Sc) | KF848582 | |||
DSS66 | F/9.9 | Abdominal pain | 2 (Sc) | KF848583 | ||
DSS67 | F/6.1 | Abdominal pain | Mixed 1 and 4 (Sc) | 0 | KF848584-5 | |
DSS68 | F/6.1 | 1 (Sc) | 0 | KF848586 | ||
DSS69 | F/6.8 | 1 | KF848587 | |||
DSS70 | F/7.8 | Abdominal pain | 3 (Sc) | KF848588 | ||
DSS71 | F/6.7 | Abdominal pain | 3 | KF848589 | ||
DSS72 | M/6 | Abdominal pain, vomiting | 3 (Sc) | KF848590 | ||
DSS73 | F/7 | Abdominal pain | 3 (Sc) | 0 | KF848591 | |
DSS74 | M/8.5 | 3 | KF848592 | |||
DSS75 | M/5.9 | Abdominal pain | 3 | KF848593 | ||
DSS76 | M/8.1 | Abdominal pain | 1 | KF848594 | ||
DSS77 | M/6.6 | Abdominal pain | 3 (Sc) | 2 to 12 | KF848595-7 | |
DSS78 | M/6.9 | Abdominal pain | 2 | KF848598 | ||
DSS79 | M/8.4 | 3 (Sc) | 0 to 11 | KF848599-600 | ||
DSS80 | M/6.1 | 3 | KF848601 | |||
DSS81 | F/5.9 | Abdominal pain | Mixed 2 and 3 (Sc) | 0 | KF848602-3 | |
DSS82 | F/9.8 | Abdominal pain | 3 (Sc) | 0 | KF848604 | |
DSS83 | M/6.4 | 1 | KF848605 | |||
DSS84 | F/9.2 | 2 (Sc) | KF848606 | |||
DSS85 | F/6.8 | Abdominal pain, vomiting | 3 (P) | 3 to 5 | KF848607-9 | |
DSS86 | F/6.6 | Abdominal pain | Mixed 1, 2 and 3 (P) | KF848610-2 | ||
DSS87 | M/9.9 | Abdominal pain | 3 (P) | 0 | KF848613 | |
DSS88 | M/9.5 | 1 (P) | 0 to 1 | KF848614-5 | ||
DSS89 | F/9.1 | Diarrhea | 3 (P) | 0 | KF848616 | |
DSS90 | F/9.5 | 3 (P) | 0 to 1 | KF848617-8 | ||
DSS91 | F/9.4 | 1 (P) | 0 to 3 | KF848619-20 | ||
DSS92 | F/6.1 | Abdominal pain, diarrhea | 3 (P) | 0 to 3 | KF848621-2 | |
DSS93 | M/6 | 3 (P) | 0 to 1 | KF848623-4 |
DNA extraction and molecular subtyping of Blastocystissp. isolates
Results and discussion
Screening for Blastocystissp. in a cohort of Senegalese children using molecular methods
Distribution of Blastocystissp. STs in the Senegalese population and comparison with other African countries
Villages |
Blastocystis sp. STs | Total no. | |||||||
---|---|---|---|---|---|---|---|---|---|
ST1 | ST2 | ST3 | ST1/ST2 | ST1/ST4 | ST2/ST3 | ST3/ST4 | ST1/ST2/ST3 | ||
Agniam Towguel
| 5 | 2 | 9 | 0 | 0 | 0 | 0 | 1 | 17 |
Niandane
| 13 | 5 | 17 | 1 | 1 | 1 | 0 | 0 | 38 |
Fanaye Diery
| 7 | 8 | 19 | 0 | 0 | 2 | 1 | 1 | 38 |
Total no.
| 25 | 15 | 45 | 1 | 1 | 3 | 1 | 2 | 93 |
Villages |
Blastocystis sp. STs (%) | Total no. | |||
---|---|---|---|---|---|
ST1 | ST2 | ST3 | ST4 | ||
Agniam Towguel | 6 | 3 | 10 | 0 | 19 |
Niandane | 15 | 7 | 18 | 1 | 41 |
Fanaye Diery | 8 | 11 | 23 | 1 | 43 |
Total no. (%) | 29 (28.1) | 21 (20.3) | 51(49.5) | 2 (1.9) | 103 |
Distribution of Blastocystissp. STs in symptomatic and asymptomatic groups
Group |
Blastocystis sp. STs (%) | Total no. | |||
---|---|---|---|---|---|
ST1 | ST2 | ST3 | Mixed infections | ||
Symptomatic | 13 (24) | 7 (13) | 28 (52) | 6 (11) | 54 |
Asymptomatic | 12 (31) | 8 (20) | 17 (44) | 2 (5) | 39 |
Total no (%)
|
25 (27)
|
15 (16)
|
45 (48)
|
8 (9)
|
93
|