Background
Materials and methods
Sample Collection
DNA Isolation
HPV Detection, Quantitation and Typing
Detection of HPV Integration by PCR
Correlation between Clinical Data and Plasma HPV DNA
Results
HPV DNA in Plasma
Total | Plasma HPV DNA | ||||
---|---|---|---|---|---|
Positive | Negative | ||||
Cervical Cancer patient | 50 | 6 | 44 | ||
Agea
| |||||
<50 | 25 | 3 | 22 | ||
>50 | 25 | 3 | 22 | ||
HPV type | |||||
Ub
| 4 | 0 | 4 | ||
16 | 28 | 2 | 26 | ||
18 | 16 | 4 | 12 | ||
33 | 2 | 0 | 2 | ||
Stage | |||||
IB | 2 | 0 | 2 | ||
IIB | 17 | 3 | 14 | ||
IIIB | 28 | 0 | 28 | ||
IVB | 1 | 1 | 0 | ||
Rc
| 2 | 2 | 0 | ||
IIB response to radiotherapy | |||||
PRd
| 3 | 2 | 1 | ||
CRe
| 15 | 1 | 13 | ||
IIB clinical outcome within | |||||
one year after complete | |||||
treatment | |||||
Rc
| 6 | 2 | 4 | ||
NEDf
| 5 | 0 | 5 | ||
Metastases | |||||
Positive | 3 | 3 | P = 0001 | 0 | |
Negative | 47 | 3 | RR = 15.67 | 44 | |
Histopathology type | |||||
Squamous | 42 | 5 | 37 | ||
Adenomatous | 8 | 1 | 7 |
Patient | Age | Stagea
| Pathology | Tumor | Response to | Time | Metastatic | HPV | Relative |
---|---|---|---|---|---|---|---|---|---|
Code | Type | Size | Radiotherapy | Recur | Organ | type | amount of | ||
(cm) | (month)b
| E6:ZP3 | |||||||
PCRf
| |||||||||
4 | 39 | IIB | Adenomatous | 7 | Partial | 18 | 0.02 | ||
148 | 65 | IIB | Squamous | 3 | Complete | 8 | Pleura, | 18 | 0.04 |
Peritoneumc
| |||||||||
183 | 27 | IIB | Squamous | 6 | Partial | 11 | Bone c
| 18 | 0.05 |
106 | 51 | IVB | Squamous | >4 | Liver, Pleurad
| 18 | 0.14 | ||
161 | 34 | Re
| Squamous | 7 | Umbillicus d
| 16 | 0.19 | ||
59 | 46 | Re
| Squamous | 5 | Liver, Lung d
| 16 | 0.05 |