Background
Methods
Cell lines
Tumor tissues
DNA extraction
Identification of MSI
Frameshift mutation analysis
Results
Frameshift mutations in CRC cell lines
Frequencies of frameshift mutations in early and late stage sporadic MSI CRCs
Patient number | Stage | Depth of invasion
a
| Tumor size | Location
b
| MSI | Mutations in mononucleotide repeat tracts | |||||
---|---|---|---|---|---|---|---|---|---|---|---|
(mm) |
TGFβRII
|
BAX
|
hMSH3
|
hMSH6
|
IGFIIR
|
Fas antigen
| |||||
1 | Early | m | 4 | A | 2/6 | -
c
| - | NI
d
| - | - | - |
2 | Early | m | 4 | A | 2/8 | - | - | NI | - | - | - |
3 | Early | m | 3 | S | 3/8 | - | - | - | - | - | - |
4 | Early | sm | 8 | R | 3/8 | - | - | - | - | - | - |
5 | Early | sm | 14 | A | 2/5 | - | - | NI | - | - | - |
6 | Early | sm | 8 | T | 3/8 | - | - | - | - | - | - |
7 | Early | sm | 4 | D | 3/10 | - | - | - | - | - | - |
8 | Early | sm | 12 | NI | 2/8 | - | - | NI | - | - | - |
9 | Early | sm | 13 | R | 2/9 | - | - | - | - | - | - |
10 | Late | mp | 9 | R | 2/8 | - | De | - | - | - | - |
11 | Late | mp | 20 | S | 3/9 | De
e
| - | De | - | - | - |
12 | Late | mp | 65 | A | 4/10 | - | - | De | De | - | - |
13 | Late | mp | 24 | S | 2/9 | - | - | - | - | - | - |
Frameshift mutation | |||
---|---|---|---|
Tumor stage | positive | negative | p value |
Early | 0 | 9 | |
Late | 3 | 1 | 0.014 |