Background
CD133, also known as prominin-1, is a five-transmembrane domain molecule [
1,
2] located on apical plasma membrane protrusions of embryonic epithelial structures [
3‐
5]. Up to now, it is mainly used for marking stem-like cells of various tissues and cancers [
6]. Many tumors are known to contain a minority population of cancer stem cells or tumor-initiating cells which have the properties of self-renewal, proliferation, and multilineage differentiation and are responsible for sustaining the tumor [
7]. Moreover, CD133 may represent a putative cancer stem cell marker in many solid tumors, such as human colon cancer [
8,
9], prostate tumor [
10,
11], pancreatic adenocarcinoma [
12], renal cancer [
13], neural tumor [
14‐
17], and hepatocellular carcinoma [
18,
19].
It has been shown that CD133 is an apical molecule not only in embryonic epithelial structures, but also in many normal human tissues and its expression is not restricted to stem cells in pancreatic tissues [
20]. In addition, CD133 has been found to be a prognostic factor of some cancers, such as colon and hepatocellular carcinoma [
21,
22]. Recently, Shimada et al. reported that normal bile duct epithelia were entirely negative for CD133 while 14 out of 29 CC cases expressed CD133. However, for each case, only a few tumor cells expressed CD133 protein; moreover, the 5-year survival rate in the CD133-positive group was worse than that in the CD133-negative group [
23]. On the contrary, in our study we detected the expression of CD133 in human CC specimens by immunohistochemistry and analyzed its correlation with clinical and histopathological features. We found that CD133 was expressed on apical membrane of normal bile ducts and apical membrane or/and in the cytoplasma of CC tissues. The positive expression of CD133 on tumor cells was significantly correlated with well or moderately differentiated CC cases and predicted a better prognosis for the patients.
Discussion
In this study, we investigated CD133 expression in normal human liver tissues and CC tissues using the immunohistochemical method. We found that CD133 was expressed in human normal livers but the expression was restricted to biliary trees on the apical membrane. In CC, 40 of 54 (74%) cases studied showed CD133 expression with different degrees. Similar to colon and mammary gland adenocarcinoma which have high expression of CD133 in well or moderately differentiated tumors [
27], most of the well or moderately differentiated CC (90% and 83%, respectively) expressed CD133 and moreover, most CD133 negative CC tumors (71%) were poorly differentiated.
Our study and other researches have found that CD133 expression pattern varies in different cancers. In colorectal carcinoma, CD133 positivity is restricted only to the luminal surface [
21,
28]. In our study, besides the luminal surface pattern in most well-differentiated CC cases, most of moderately or poorly differentiated CC cases showed cytoplasmic or both cytoplasmic and membranous staining (Table
2). It is believed that the different subcellular localization of CD133 (cytoplasm or membrane) may confer different cellular functions [
20]. The protein is expressed apically on the membrane of epithelial cells when a lumen has been formed but it is expressed in the cytoplasm of solidly arranged malignant tissues of non-epithelial origin [
20]. It is well known that if a formed lumen is observed in an epithelial tumor, it usually indicates for better differentiation of the glandular tumor. In our study, CD133 showed cytoplasmic expression in many CC cases, suggesting that cytoplasmic CD133 expression should be not just restricted to malignant tissues of non-epithelial origin [
20] but also occurs in malignant epithelial cells of CC. The statistical analysis showed that CD133 expression significantly correlated with the differentiation status of CC (
P = 0.004). It is possible that the expression of CD133 on the apical membrane may indicate a particular stage of the cell differentiation connected with the formation of lumen and ducts as same as the normal bile ducts, and this deduction is confirmed by Shmelkov, S.V. et al in their study [
28]. In addition, we noticed the CD133 positive debris in normal bile ducts and the shedding of CD133 positive tumor cells and apparent non-cellular, CD133-positive materials in the malignant ducts. This observation is in accordance with the previous report that small membrane vesicles containing prominin-1 were found in the human normal saliva and tears as well as malignant lumen of colorectal cancer [
29,
30]. The concentration of CD133, presented within the apical membrane of glands or in extracellular membranous vesicles, suggests that this cholesterol-binding glycoprotein might play a role in regulating the secreting process [
29,
31]. Similarly, CD133 expression in normal bile ducts and its immunoreactivity associated with the secretion therein indicate that CD133 protein might exist in bile, thus, a quantitive analysis of CD133 in bile might be helpful for distinguishing CC from hepatocellular carcinoma.
Many previous researches have demonstrated the importance of CD133 as a defining factor of the cancer stem cell phenotype, including human liver cell line [
19] and hepatocellular carcinoma [
32]. In this study, however, we found that CD133 was commonly expressed on the apical membrane of all normal bile ducts and was frequently expressed in the majority of CC cases, which were clearly at the differentiation stages beyond the stem/progenitor cells. Other studies also find that the overall expression of human CD133 is beyond the rare primitive cells and appears to be a general marker for apical membrane of fully differentiated glandular epithelia [
4,
20,
28,
33]. These findings suggest that it should be inappropriate to employ CD133 as morphological characterization of cancer stem cells and the value of CD133 expression as a marker for cancer stem cells should be critically evaluated in future studies [
34].
Recently, several studies have reported that the presence of CD133 in various tumors is correlated with poor prognosis. Zeppernick [
35] and Beier [
17] have found that CD133 expression indicates shorter survival for adverse gliomas and high-grade oligodendroglial tumors. Similarly, the high expression of CD133 in colorectal adenocarcinoma is related to poor survival of the patients [
21,
25]. Furthermore, the high expression of CD133 is correlated with the increased tumor grade, advanced disease stage, elevated serum alpha-fetoprotein levels and poor survival of the patients with hepatocellular carcinoma [
21]. In this study, we found that CD133 expression is also significantly associated with the survival of the patients with CC. However, there are differences between our findings and the previous reports. Firstly, the patients of CC with positive CD133 expression had significantly better prognosis than those negative ones. Secondly, the patterns of CD133 expression varied in different human CC cases and seemed to be related to differentiation stages of the tumors. Like glandular epithelia that usually show apical membrane expression pattern of CD133 [
20,
33], normal bile ducts showed the same expression pattern. Interestingly, similar expression pattern of CD133 was found in well-differentiated CC. In contrast, poorly differentiated CC cases usually showed solid growth pattern and most of them were cytoplasmic positive or even negative for CD133. Nevertheless, in the patients with pancreatic ductal adenocarcinoma, there is no correlation between the level of CD133 expression and patient survival [
20]. However, Maeda S et al found that the 5-year survival rate of CD133-positive patients was significantly lower than that of CD133-negative patients [
36].
Therefore, the role of CD133 expression in evaluating prognosis of cancer patients needs to be explored in further work. Until now, the physiological role of CD133 is still elusive probably due to the occurrence of many mRNA splice variants and the changing glycosylation status of the protein [
37,
38]. Moreover, the protein has different subcelluar localizations (membrane, cytoplasm or both), which may confer different functions. It also remains to investigate why CD133 is usually present on apical membrane of normal bile ducts or other gland lumen, why it is transferred from apical membrane to cytoplasm in cancer cells and what its biological functions in normal bile duct epithelium and cholangiocacinoma are. And the answers to these questions could help us to understand the role of CD133 in the development of CC and other tumors.
Conclusions
We found CD133 expressed mainly on the apical membrane surface of bile ducts of all sizes and most of malignant ducts. And the subcellular localization of the CD133 protein was investigated and three staining patterns, i.e. apical membrane, cytoplasm or both, were presented in CC and the first pattern was frequently found in well-differentiated CC. In addition, the positive expression of CD133 was significantly correlated with well or moderately differentiated CC and vice versa. Moreover, the patients of CC with positive CD133 expression had a significantly better prognosis than those negative ones. In brief, our study demonstrates that CD133 expression correlates with the differentiation of CC and indicates that CD133 is a potential indicator for differentiation and prognosis of human CC, the mechanisms of which, however, need to be investigated in further study.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
LF performed the statistical analysis and drafted the manuscript. FH carried out immunohistochemistry staining and drafted the manuscript. HL helped to revise the draft. JZ and YL participated in collecting the samples. ZY carried out the figures and tables. LW and YG participated in the follow-up study. ZW and QY helped to confirm the diagnosis of the samples. GH conceived the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.