Background
Methods
Subjects
Clinical measurements
Genotyping
Statistical analysis
Results
Baseline characteristics
Characteristic | Diabetic cases (n = 1118) | Non-diabetic controls (n = 1161) | P value* |
---|---|---|---|
Age (years) | 60.2 ± 0.37 | 56.5 ± 0.32 | <0.001 |
Men (%) | 44.6 | 42.7 | 0.34 |
BMI (kg/m2) | 24.4 ± 0.11 | 23.5 ± 0.09 | <0.001 |
Fasting glucose (mmol/l) | 8.17 ± 0.04 | 4.86 ± 0.01 | <0.001 |
Total cholesterol (mmol/l) | 4.37(3.78-5.07) | 5.10(4.50-5.80) | <0.001 |
Triglyceride (mmol/l) | 1.47(1.00-2.24) | 1.40(1.00-1.90) | <0.001 |
HDL-C (mmol/l) | 1.12(1.00-1.38) | 1.30(1.12-1.54) | <0.001 |
LDL-C (mmol/l) | 2.38(1.92-3.01) | 3.07(2.50-3.60) | <0.001 |
Associations of MTNR1B polymorphisms with type 2 diabetes
SNP identification | Chromosome Position | Relation to the gene | Major allele | Minor allele | MAF | HWE P value | |
---|---|---|---|---|---|---|---|
1 | rs10830963 | 92348358 | Intron | C | G | 0.40 | 0.13 |
2 | rs3781637 | 92353418 | Intron | A | G | 0.14 | 0.13 |
3 | rs1562444 | 92355497 | 3'UTR | A | G | 0.31 | 0.14 |
SNP | Alleles Major/minor | MAF | Genotype Frequency T2DM cases/controls | ORadd (95%CI) a | P value* | ORdom (95%CI)b | P value* | ORrec (95%CI) c | P value* |
---|---|---|---|---|---|---|---|---|---|
rs10830963 | C/G | 0.40 | 0.361/0.481/0.159 | 0.92 | 0.22 | 0.85 | 0.08 | 1.01 | 0.96 |
0.348/0.508/0.144 | (0.81-1.05) | (0.70-1.02) | (0.78-1.30) | ||||||
rs3781637 | A/G | 0.14 | 0.717/0.260/0.022 | 1.22 | 0.038 | 1.17 | 0.13 | 2.81 | 0.01 |
0.736/0.252/0.012 | (1.01-1.46) | (0.96-1.43) | (1.28-6.17) | ||||||
rs1562444 | A/G | 0.31 | 0.472/0.432/0.096 | 1.06 | 0.42 | 1.02 | 0.83 | 1.27 | 0.14 |
0.457/0.456/0.088 | (0.92-1.22) | (0.85-1.22) | (0.92-1.74) |
Associations of MTNR1B polymorphisms with quantitative traits in non-diabetic controls
rs10830963 | |||||||||
---|---|---|---|---|---|---|---|---|---|
Genotype
|
CC
|
CG
|
GG
|
β
add
± SEM
a
|
P
add
a
*
|
β
dom
± SEM
b
|
P
dom
b
*
|
β
rec
± SEM
c
|
P
rec
c
*
|
n | 396 | 578 | 164 | ||||||
Fasting glucose (mmol/l) | 4.86 ± 0.02 | 4.87 ± 0.02 | 4.84 ± 0.03 | -0.009 ± 0.017 | 0.59 | 0.0004 ± 0.024 | 0.99 | -0.033 ± 0.032 | 0.30 |
Triglyceride (mmol/l) | 1.40(1.10-1.90) | 1.40(1.00-1.90) | 1.40(1.10-1.80) | -0.062 ± 0.049 | 0.21 | -0.079 ± 0.070 | 0.25 | -0.080 ± 0.094 | 0.40 |
Total cholesterol (mmol/l) | 5.20(4.50-5.90) | 5.10(4.50-5.73) | 5.10(4.50-5.80) | -0.015 ± 0.045 | 0.74 | -0.042 ± 0.063 | 0.50 | 0.022 ± 0.084 | 0.79 |
LDL-C (mmol/l) | 3.10(2.54-3.64) | 3.09(2.50-3.57) | 3.09(2.57-3.67) | -0.002 ± 0.039 | 0.97 | -0.020 ± 0.055 | 0.72 | 0.030 ± 0.075 | 0.69 |
HDL-C (mmol/l) | 1.30(1.10-1.60) | 1.40(1.13-1.53) | 1.40(1.14-1.50) | -0.003 ± 0.014 | 0.84 | -0.011 ± 0.019 | 0.57 | 0.010 ± 0.026 | 0.70 |
rs3781637
| |||||||||
Genotype
|
AA
|
AG
|
GG
|
β
add
± SEM
a
|
P
add
a
*
|
β
dom
± SEM
b
|
P
dom
b
*
|
β
rec
± SEM
c
|
P
rec
c
*
|
n | 841 | 288 | 14 | ||||||
Fasting glucose (mmol/l) | 4.87 ± 0.01 | 4.84 ± 0.02 | 4.78 ± 0.10 | -0.037 ± 0.025 | 0.14 | -0.035 ± 0.026 | 0.18 | -0.129 ± 0.124 | 0.30 |
Triglyceride (mmol/l) | 1.40(1.00-1.90) | 1.40(1.00-1.90) | 1.70(1.13-2.18) | 0.032 ± 0.071 | 0.65 | 0.002 ± 0.075 | 0.98 | 0.742 ± 0.314 | 0.018 |
Total cholesterol (mmol/l) | 5.10(4.50-5.80) | 5.10(4.40-5.80) | 5.60(5.40-6.38) | -0.022 ± 0.065 | 0.74 | -0.062 ± 0.069 | 0.367 | 0.841 ± 0.316 | 0.008 |
LDL-C (mmol/l) | 3.07(2.55-3.62) | 3.02(2.50-3.56) | 3.36(2.81-4.26) | -0.021 ± 0.057 | 0.71 | -0.050 ± 0.060 | 0.40 | 0.585 ± 0.281 | 0.038 |
HDL-C (mmol/l) | 1.30(1.13-1.53) | 1.30(1.11-1.60) | 1.38(1.17-1.59) | -0.001 ± 0.020 | 0.95 | 0.003 ± 0.021 | 0.89 | -0.086 ± 0.095 | 0.36 |
rs1562444
| |||||||||
Genotype
|
AA
|
AG
|
GG
|
β
add
± SEM
a
|
P
add
a
*
|
β
dom
± SEM
b
|
P
dom
b
*
|
β
rec
± SEM
c
|
P
rec
c
*
|
n | 520 | 518 | 100 | ||||||
Fasting glucose (mmol/l) | 4.86 ± 0.02 | 4.86 ± 0.02 | 4.87 ± 0.04 | 0.005 ± 0.018 | 0.79 | 0.005 ± 0.023 | 0.83 | 0.009 ± 0.042 | 0.83 |
Triglyceride (mmol/l) | 1.40(1.00-1.90) | 1.40(1.00-1.90) | 1.40(0.90-1.90) | 0.009 ± 0.047 | 0.85 | 0.046 ± 0.060 | 0.44 | -0.108 ± 0.109 | 0.32 |
Total cholesterol (mmol/l) | 5.10(4.50-5.80) | 5.10(4.50-5.80) | 5.10(4.40-5.90) | -0.004 ± 0.047 | 0.94 | -0.019 ± 0.060 | 0.75 | 0.046 ± 0.110 | 0.67 |
LDL-C (mmol/l) | 3.11(2.57-3.64) | 3.03(2.50-3.60) | 3.00(2.59-3.58) | -0.022 ± 0.042 | 0.60 | -0.041 ± 0.053 | 0.44 | 0.020 ± 0.097 | 0.84 |
HDL-C (mmol/l) | 1.30(1.13-1.51) | 1.30(1.11-1.54) | 1.36(1.16-1.65) | 0.010 ± 0.014 | 0.51 | 0.001 ± 0.018 | 0.97 | 0.049 ± 0.033 | 0.14 |