Introduction
Methods
Subjects
Experimental protocols
Echocardiography
Euthanasia and Histopathological Methods
Electron microscopy
Statistical Analysis
Results
1) Establishment of diabetic cardiomyopathy
LETO (-) (n = 8) | OLETF (-) (n = 7) | OLETF (+) (n = 12) | p | |
---|---|---|---|---|
BW (g)
|
428 ± 18.8
|
551.4 ± 44.9*
|
595.7 ± 37.5*‡
|
< 0.001
|
FBG (mg/dl)
|
125.4 ± 9.8
|
148.0 ± 28.5
|
151.3 ± 34.5
|
0.129
|
TC (mg/dl)
|
71.9 ± 19.8
|
97.3 ± 25.0**
|
89.8 ± 15.1
|
0.045
|
TG (mg/dl)
|
46.6 ± 23.4
|
197.7 ± 91.2**
|
190.7 ± 41.6*
|
< 0.001
|
HR (bpm)
|
312.9 ± 18.7
|
297.6 ± 24.5
|
298.8 ± 12.2
|
0.177
|
E (cm/sec)
|
72.8 ± 3.3
|
66.3 ± 1.7**
|
65.5 ± 5.4*
|
0.002
|
E' (cm/sec)
|
9.0 ± 1.4
|
6.7 ± 0.2*
|
5.2 ± 0.3*†
|
< 0.001
|
E/E'
|
8.2 ± 1.0
|
9.8 ± 0.4*
|
12.5 ± 0.8*†
|
< 0.001
|
DT (msec)
|
81.8 ± 12.3
|
83.6 ± 9.5
|
86.3 ± 11.4
|
0.675
|
2) G-CSF improves cardiac diastolic function
LETO (-) (n = 8) | OLETF (-/-) (n = 4) | OLETF (-/+) (n = 2) | p | |
---|---|---|---|---|
BW (g)
|
444.0 ± 15.2
|
600.2 ± 31.2*
|
537.5 ± 95.5**
|
< 0.001
|
FBG (mg/dl)
|
210.8 ± 46.6
|
364.0 ± 120.0**
|
281.0 ± 89.1
|
0.025
|
TC (mg/dl)
|
55.4 ± 6.9
|
61.5 ± 7.6
|
61.0 ± 12.7
|
0.397
|
TG (mg/dl)
|
45.9 ± 12.5
|
151.0 ± 62.0*
|
162.0 ± 46.7*
|
0.001
|
HR (bpm)
|
292.9 ± 18.1
|
283.5 ± 28.3
|
291.0 ± 24.0
|
0.785
|
E (cm/sec)
|
79.5 ± 4.2
|
73.6 ± 2.2
|
75.2 ± 0.4
|
0.051
|
E' (cm/sec)
|
7.1 ± 0.5
|
5.7 ± 0.3*
|
6.7 ± 0.4
|
0.001
|
E/E'
|
11.2 ± 0.6
|
12.9 ± 0.7*
|
11.3 ± 0.7‡
|
0.004
|
DT (msec)
|
76.4 ± 5.5
|
68.5 ± 12.8
|
73.5 ± 0.7
|
0.311
|
BP (mmHg)
|
160.0 ± 14.1
|
167.2 ± 10.1
|
167.4 ± 34.8
|
0.950
|
LETO (-) (n = 8) | OLETF (+/-) (n = 6) | OLETF (+/+) (n = 6) | p | |
---|---|---|---|---|
BW (g)
|
444.0 ± 15.2
|
624.0 ± 23.8*
|
598.7 ± 50.8*
|
< 0.001
|
FBG (mg/dl)
|
210.8 ± 46.6
|
367.3 ± 47.9*
|
314.0 ± 56.2*
|
< 0.001
|
TC (mg/dl)
|
55.4 ± 6.9
|
58.5 ± 9.7
|
54.2 ± 16.5
|
0.790
|
TG (mg/dl)
|
45.9 ± 12.5
|
116.3 ± 16.4*
|
128.2 ± 55.9**
|
< 0.001
|
HR (bpm)
|
292.9 ± 18.1
|
291.7 ± 35.5
|
289.5 ± 18.6
|
0.968
|
E (cm/sec)
|
79.5 ± 4.2
|
70.1 ± 1.4*
|
73.9 ± 3.3**
|
0.001
|
E' (cm/sec)
|
7.1 ± 0.5
|
4.7 ± 0.1*
|
6.6 ± 0.2†
|
< 0.001
|
E/E'
|
11.2 ± 0.6
|
15.0 ± 0.7*
|
11.3 ± 0.8†
|
< 0.001
|
DT (msec)
|
76.4 ± 5.5
|
69.3 ± 7.7
|
72.0 ± 4.0
|
0.105
|
BP (mmHg)
|
160.0 ± 14.1
|
166.9 ± 2.0
|
177.0 ± 21.9
|
0.587
|
OLETF (+/-) | OLETF (+/+) | |||||
---|---|---|---|---|---|---|
Pre- treatment | Post- treatment | p | Pre- treatment | Post- treatment | p | |
E (cm/sec)
|
68.7 ± 6.1
|
70.1 ± 1.4
|
0.611
|
62.4 ± 1.8
|
73.9 ± 3.3
|
0.001
|
E' (cm/sec)
|
5.3 ± 0.3
|
4.7 ± 0.1
|
0.006
|
5.2 ± 0.3
|
6.6 ± 0.2
|
0.001
|
E/E'
|
13.0 ± 0.5
|
15.0 ± 0.7
|
0.001
|
12.0 ± 0.6
|
11.3 ± 0.8
|
0.224
|