Allelic dimorphism of the erythocyte binding antigen-175 (eba-175) gene of Plasmodium falciparum and severe malaria: Significant association of the C-segment with fatal outcome in Ghanaian children

Malaria parasites invade host erythrocytes via interaction between merozoite surface ligands and erythrocyte receptors [1, 2]. In Plasmodium falciparum, the erythrocyte binding antigen-175 (EBA-175) is one of the most important ligands mediating erythrocyte invasion by sialic acid dependent binding to glycophorin A [3, 4]. The 175 kDa protein is located in micronemes at the apical end of the merozoite [5]. Together with the Duffy-binding protein of Plasmodium vivax (PvDBP), EBA-175 belongs to a family of parasite adhesion molecules, the Duffy-binding-like erythrocyte-binding proteins (DBL-EBP) [6]. This protein family is characterized by a N-terminal signal peptide, cystein-rich Duffy-binding-like (DBL) domains, a carboxyl cysteine-rich region (c-cys), a transmembrane domain and a short cytoplasmatic tail. Encoding genes are members of the erythrocyte-binding-like (ebl) gene family. The eba-175 gene is located on chromosome 7 and consists of four exons [7, 8]. A large exon 1 is divided into regions I-VI and codes for a tandem array of two DBL domains in region II (Fl and F2) forming the putative ligand site as well as the c-cys domain, region VI. Both domains have numerous conserved cystein and hydrophobic amino acid residues supporting their functional relevance. They are separated by highly divergent dimorphic segments in region III [9]. This dimorphism is characterized by an insertion of either a 342 bp segment in FCR-3 strains (F-segment) or a 423 bp segment in CAMP strains (C-segment) [7, 10]. Both segments share little homology and are located at slightly different positions with the F-segment being 273 bp upstream of the C-segment. These two variants are conserved in all strains isolated so far and since Plasmodium merozoites are haploid either one or the other, but never both or neither segment is present in one individual parasite clone [10]. The functional relevance of the F-/C-segment dimorphism remains unknown. Human IgG antibodies recognize epitopes on the dimorphic F- and C-segments but are not associated with protection from clinical malaria in The Gambia [11]. Population genetic analysis showed a C-segment allele frequency of 73% in eba-175 of P. falciparum in a Sudanese population whereas more than 70% of isolates from The Gambia, Nigeria, Cameroon, Gabon, Tanzania and South Africa carried the F-segment [12, 13].

The median age of the 289 children with severe malaria (154 girls, 135 boys) was 24 months (range 6–102). Mean rectal temperature (±SD) was 38.6°C (±1.1). GMPD was 30,200/μL (95% CI, 22,474–40,581) and hyperparasitaemia (>250,000/μL) was present in 64 children (22%). The GMPD of parasitaemic controls was 1,738/μL (95% CI, 1,387–2,178). Cases exhibited the following symptoms defining severe malaria (WHO 2000): severe anemia (55%), prostration (34%), respiratory distress (23%), multiple convulsions (20%), impaired consciousness (19%), jaundice (12%), circulatory collapse (3%), and haemoglobinuria (3%). Case fatality rate was 11% (32/284). For five children, no data on outcome were available since they absconded during hospitalization.

In children with severe malaria, the F- and C-segment were present in 70% (201/289) and 19% (55/289), respectively (see Table 1). Both segments were found in 11% (33/289) representing mixed infections. In the parasitaemic controls, the prevalences for the F- and C-segment were 61% (176/289) and 17% (49/289), respectively. Mixed infections were significantly less frequent in severe cases than in parasitaemic controls (11% (33/289) versus 22% (64/289); OR .5, [95% CI .3–.7], P = .003; F-segment used as reference). Matched pair analysis revealed no significant associations of the C- or F-segment with individual symptoms and conditions of SM (Table 1). However, for all symptoms and conditions of SM except for haemoglobinuria, the C-segment was more common in severe cases while mixed infections occured more often in respective parasitaemic controls (not significant).

A trend towards a higher risk of dying of severe malaria was found in children with the C-segment in matched pair analysis (OR 4.0, [95% CI .9–19.4], P = .1; Table 1). Associations of the eba-175 dimorphism with symptoms, conditions, and outcome within the group of severe malaria cases were re-tested (Figure 1). The C-segment was significantly associated with fatal outcome (case fatality rates: F-segment: 8%, F-/C-segment: 13%, C-segment: 24%; _²_trend = 11.3, P = .0008). Logistic regression analysis adjusting for known predictors of death in these children [15] confirmed a six-fold increased risk of fatal outcome in patients infected with C-segment parasites as compared to F-segment parasites (OR 5.6, [95% CI 1.8–17.1], P < .003; other predictors were: impaired consciousness, OR, 9.3; 95% CI, 3.4–25.9; P < .0001; circulatory collapse, OR, 35.9; 95% CI, 4.5–290; P = .0008; hypoglycemia, OR, 3.7; 95% CI, 1.3–10.3; P = .01; malnutrition, OR, 95% CI, 2.5; 1.0–6.3; P = .06).

This study aimed at evaluating the frequencies of a dimorphic allele segment in the gene encoding the erythrocyte binding antigen-175 on P. falciparum merozoites in children with severe malaria in northern Ghana. Second, associations of the eba-175 dimorphism with symptoms, conditions, and outcome of severe disease were assessed in a case-control analysis.

In 289 Ghanaian children with severe malaria, the F-segment predominated (70%). C-segment and mixed infections were present in 19% and 11%, respectively. These findings are consistent with recently published data from West Africa [13].

In the case-control analysis, mixed infections were significantly more common in controls than in cases. This is in accordance with findings from other areas with high transmission [19, 20] and is considered to indicate acquired immunity [21]. However, the presence of either the F- or the C-segment alone does not exclude the possibility of infection with different strains and, therefore, limits validity of this finding. The overall distribution of the F- and C-alleles did not differ significantly between groups arguing against a role for the eba dimorphism in mediating severe malaria per se. This is consistent with the finding in the Gambia that antibodies against the dimorphism do not protect against clinical malaria [11]. Yet, within the group of children with severe malaria, fatal outcome was significantly associated with the C-segment (Figure 1). These findings suggest that the C-segment of the eba-175 dimorphism on P. falciparum merozoites is associated with fatality in severe malaria rather than promoting disease progression from asymptomatic parasitaemia towards severe disease. A longitudinal study is needed to confirm this.

Unlike in West Africa, in a Sudanese population the C-segment was most frequent [13]. This part of Africa represents an ethnically distinct region with lower malaria endemicity. In a study conducted in Laos, the dimorphism was unequally distributed in distinct ethnic minorities [18]. Random shifts in parasite allele frequencies in different geographic regions can serve as an explanation. Also, susceptibility to infection and disease severity vary in different regions as does genetic variability in host populations. On the other hand, not only genetic differences in the host but also in the parasite may influence disease severity and outcome. Although the functional mechanism of the eba-175 dimorphism regarding disease severity remains unclear one might postulate that this dimorphism offers alternative ways of erythrocyte invasion to the parasite and therefore allows more rapid replication. Since the eba-175 dimorphism appears not to be involved in the binding to neuraminidase- or trypsin-sensitive erythrocyte receptors [22] it may recognize other, as yet unknown, receptors. This explanation is supported by the finding that the C-segment was associated at borderline statistical significance with hyperparasitaemia (OR 3.7 [1.1–13.1] P = .06. see Table 1).

In conclusion, the C-segment of the eba-175 allelic dimorphism on P. falciparum merozites is not associated with severe malaria per se but confers a higher risk of fatal disease in northern Ghana. These findings may contribute to the development of a diagnostic tool predicting disease outcome in children. Further studies are necessary to reveal its functional relevance.