Background
Methods
In vitro culture of Plasmodium falciparum
Parasitized blood from patients
Purification
Results and discussions
Concentration and synchronization of P. falciparum schizonts
Parasitaemia (%)a | |||
---|---|---|---|
Strain | Initialc | Plasmion® | Magnetic columns |
FcB1 (K+)b | 2.2 | 14.8 (6.7)c | 98 (44) |
FcM29 (K-) | 2.8 (Fig. A) | 3.4 (1.2). | 96 (34) (Fig. B) |
F32 (K+) | 3.1 | 13 (4.2) | 91 (29) |
W2 (K+) | 2.1 | 11 (5.2) | 94 (44) |
Concentration of P. falciparum gametocytes
Initiala | Eluentb | Final countc | Enrichment rated | |
---|---|---|---|---|
Patients bloode | 21 | 2 | 1120 | 53 |
6 | 1 | 514 | 85 | |
19 (Fig. C) | 1 | 819 (Fig. D) | 43 | |
In vitro culture | 0.8 ± 0.03%f | < 0.01 | 91% | 114 |
Stage II–III | 0.9 ± 0.1% | < 0.01 | 88% | 98 |
In vitro culture Stage IV–V | 0.4 ± 0.02% | < 0.01 | 95% | 237 |
0.8 ± 0.1% | < 0.01 | 92% | 115 | |
0.7% ± 0.1% | < 0.01 | 94% (Fig. 1-E) | 134 |
Non-falciparum purification
Parasitaemia (%) | |||
---|---|---|---|
Species | Initial: | After magnetic columns | |
P. malariae
a
| Trophozoites and schizonts | < 0.1 | 12 |
0.3 (Fig. G) | 17 (Fig. H)b | ||
P. ovale
| Trophozoites and schizonts | < 0.1 | 15 |
Trophozoites schizonts and gametocytes | <0.01 and presence of gametocytes | 6, and numerous gametocytes (Fig. F) | |
P. vivax | Trophozoites and schizonts | < 0.1 | 16 |