Background
Methods
Parasites, animals, and reagents
Small RNA preparation, cloning and analysis
Northern blot analysis
Synthesis and administration of miR-451 antisense oligos (ASO)
Results
Cloning of short RNAs from P. falciparum-infected RBCs
miRNAs | Number of clones | Percentage(%) |
---|---|---|
hsa-let-7a
| 1 | 2.78 |
hsa-let-7b
| 7 | 19.44 |
hsa-let-7f
| 1 | 2.78 |
hsa-mir-16
| 6 | 16.67 |
hsa-mir-91
| 2 | 5.56 |
hsa-mir-92
| 1 | 2.78 |
hsa-mir-106
| 1 | 2.78 |
hsa-mir-142
| 2 | 5.56 |
hsa-mir-144
| 2 | 5.56 |
hsa-mir-451
| 14 | 38.89 |
Total
| 36 | 100 |