Background
Methods
Cell culture and tumour model
Gene expression data collection and analysis
Protein expression data collection and analysis
Combined expressional analysis of overlapping pathways
Results
Knockdown of STAT1 in SCC61 tumour xenografts leads to growth suppression and radio sensitization
Identification of downstream functions of STAT1 using combined transcriptomic-proteomic expressional analysis
Pathway |
P-value* (gene) |
P-value* (protein) |
---|---|---|
Oxidative phosphorylation | 0.00251 | 0.0955 |
Citrate cycle | 0.00741 | 0.0182 |
Urea cycle and metabolism of amino groups | 0.00794 | 0.0224 |
Pyruvate metabolism | 0.0380 | 0.000151 |
Nitric oxide signaling in the cardiovascular system | 0.0389 | 0.0245 |
Glycolysis/gluconeogenesis | 0.0589 | 0.000000000776 |
STAT1 modulates the expression of genes encoding proteins involved in glycolysis, oxidative phosphorylation and the citrate cycle
Pathway | Gene Symbol | Description | Function |
---|---|---|---|
GG | ALDH1A1 | Aldehyde dehydrogenase 1 family, member A1 | Oxidation of acetaldehyde to acetate |
GG | ALDH2 | Aldehyde dehydrogenase 2 family (mitochondrial) | Oxidation of acetaldehyde to acetate |
GG | ALDOA | Aldolase A, fructose-bisphosphate | Cleavage of fructose 1,6-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate |
GG | ENO1 | Enolase 1, (alpha) | Dehydration of 2-phosphoglycerate to phosphoenolpyruvate |
GG | ENO2 | Enolase 2 (gamma, neuronal) | Dehydration of 2-phosphoglycerate to phosphoenolpyruvate |
GG | ENO3 | Enolase 3 (beta, muscle) | Dehydration of 2-phosphoglycerate to phosphoenolpyruvate |
GG | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | Oxidation and phosphorylation of glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate with reduction of NAD+ to NADH |
GG | GPI | Glucose phosphate isomerase | Conversion of glucose 6-phosphate to fructose 6-phosphate |
GG | LDHA | Lactate dehydrogenase A | Reduction of pyruvate to lactate with oxidation of NADH to NAD+ |
GG | LDHAL6B | Lactate dehydrogenase A-like 6B | Reduction of pyruvate to lactate with oxidation of NADH to NAD+ |
GG | LDHB | Lactate dehydrogenase B | Reduction of pyruvate to lactate with oxidation of NADH to NAD+ |
GG | PGAM1 | Phosphoglycerate mutase 1 (brain) | Conversion of 3-phosphoglycerate to 2-phosphoglycerate |
GG | PGK1 | Phosphoglycerate kinase 1 | Phosphoryl transfer from 1,3-bisphosphoglycerate to ADP with formation of 3-phosphoglycerate and ATP |
GG | PGM1 | Phosphoglucomutase 1 | Interconversion of glucose 1-phosphate and glucose 6-phosphate |
GG | PKM2 | Pyruvate kinase type 2, muscle | Phosphoryl transfer from phosphoenolpyruvate to ADP with formation of pyruvate and ATP |
GG | TPI1 | Triosephosphate isomerase 1 | Interconversion of dihydroxyacetone phosphate and glyceraldehyde 3-phosphate |
OP | ATP5A1 | ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle | Synthesis of ATP from ADP and inorganic phosphate |
OP | ATP5O | ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit | Synthesis of ATP from ADP and inorganic phosphate |
OP | ATP5B | ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide | Synthesis of ATP from ADP and inorganic phosphate |
OP | COX4I1 | Cytochrome c oxidase subunit IV isoform 1 | Oxidation of cytochrome C with reduction of O2 to H2O |
OP/CC | SDHA | Succinate dehydrogenase complex, subunit A, flavoprotein (Fp) | Oxidation of succinate to fumarate with reduction of FAD+ to FADH2 |
CC | MDH2 | Malate dehydrogenase 2, NAD (mitochondrial) | Oxidation of malate to oxaloacetate with reduction of NAD+ to NADH |